【摘要】 目的：建立气相色谱-质谱联用法测定酱油中3-氯-1,2-丙二醇（3-Chloro-1,2-Propanediol,3-MCPD）的方法。方法：采用Extrelut^TM20柱为固相萃取柱，40 mL乙酸乙酯为洗脱溶剂进行试样净化和衍生化，通过DB-5MS石英毛细管柱分离，全扫描模式检测。结果：3-MCPD在0～200 ng·mL^-1线性关系良好，检出限为0.248μg·L^-1，加标回收率为103%～113%，日内相对标准偏差为1.9%～4.9%，日间相对标准偏差为2.2%～6.2%。结论：本方法在测定酱油中3-MCPD时，具有时效性和安全性高、灵敏度高、结果准确可靠等优点，适用于实验室环境中对酱油中3-MCPD含量的监测。更多还原

【Abstract】 Objective:To establish a gas chromatography-mass spectrometry method for the determination of3-chloro-1,2-propanediol （3-MCPD） in soy sauce.Method:The Extrelut^TM20 column was used as the solid phase extraction column,and 40 mL of ethyl acetate was used as the elution solvent for sample purification and derivatization.The samples were separated using a DB-5MS quartz capillary column and detected in full scan mode.Result:3-MCPD showed a good linear relationship from 0 ng·mL^-1 to 200 ng·mL^-1,the detection limit is 0.248μg·L^-1,the spiked recovery rate is 103%to113%,the intra day relative standard deviation is 1.9%to 4.9%,and the inter day relative standard deviation is 2.2%to 6.2%.Conclusion:This method has the advantages of high timeliness and safety,high sensitivity,accurate and reliable results in the determination of 3-MCPD in soy sauce,and is suitable for monitoring the content of 3-MCPD in soy sauce in laboratory environments.更多还原