【摘要】 本研究比较8个茯苓菌株胞外代谢产物和体外抗氧化活性的差异，并采用主成分分析（principal componentsanalysis,PCA）法对茯苓菌株进行综合评价。使用广泛靶向代谢组学分析胞外黄酮类和胞外酚类化合物的组成，研究茯苓发挥抗氧化作用的主要成分。结果表明，茯苓GTR1菌株胞外多糖、胞外三萜和胞外甾醇含量最高；茯苓GTR2菌株胞外黄酮和胞外总酚含量最高。体外抗氧化实验结果表明，茯苓F5.78菌株对2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基的清除能力最高，茯苓GTR2菌株的1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基清除率最高，茯苓XJ28菌株的羟自由基清除率最高。相关性分析结果表明，茯苓菌株的DPPH自由基清除率、胞外总酚含量与胞外黄酮含量呈极显著正相关（P<0.01）。通过超高效液相色谱-质谱联用仪定性检测出55种黄酮类化合物和33种酚类化合物，不同茯苓菌株之间各组分的相对含量存在差异，其中茯苓FLA、FGD、FGZ、FHB、GTR1、XJ28和F5.78菌株相对含量最高的黄酮类组分是丙二酰大豆黄酮，相对含量最高的酚类组分是3-醛基水杨酸。茯苓GTR2菌株相对含量最高的黄酮类组分是山柰酚-3-O-(6-没食子酰)葡萄糖苷，该组分相对含量与DPPH自由基清除率呈极显著正相关（P<0.01），相对含量最高的酚类组分为α-氰基-4-羟基肉桂酸，该组分与DPPH自由基清除率呈显著正相关（P<0.05）。根据PCA评价，综合得分最高的是GTR2菌株。GTR2菌株胞外活性成分含量较高、抗氧化活性好，可作为茯苓抗氧化产品开发的潜力菌株。更多还原

【Abstract】 This study examined the differences in the extracellular metabolites and in vitro antioxidant activity of 8 strains of Wolfiporia hoelen(Fr.) Y.C. Dai & V. Papp and conducted their comprehensive evaluation using principal component analysis(PCA). To investigate the major antioxidant components of W. hoelen, widely targeted metabolomics was used to analyze the composition of extracellular flavonoids and phenolic compounds. The results showed that the contents of extracellular polysaccharides, total triterpenes and sterols were the highest in strain GTR1. The contents of extracellular flavonoids and phenolics were the highest in strain GTR2. The results of in vitro antioxidant tests showed that strains F5.78, GTR2 and XJ28 had the highest capacity to scavenge 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation, 1,1-diphenyl-2-picrylhydrazyl(DPPH) and hydroxyl radicals, respectively. The results of correlation analysis showed that the contents of extracellular flavonoids was highly significantly positively correlated with the DPPH radical scavenging capacity and the contents of extracellular total phenols in W. hoelen(P < 0.01). Totally 55 flavonoids and 33phenolic compounds were qualitatively detected using ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS). The relative content of each component varied among different W. hoelen strains; the most abundant flavonoid was malonyldaidzin and the most abundant phenolic component was 3-formylsalicylic acid in W. hoelen strains FLA,FGD, FGZ, FHB, GTR1, XJ28 and F5.78. The most abundant flavonoid component in strain GTR2 was kaempferol-3-O-(6-galloyl)glucoside, which was highly significantly positively correlated with the DPPH radical scavenging capacity(P < 0.01). In this strain, the most abundant phenolic component was α-cyano-4-hydroxycinnamic acid, which was significantly positively correlated with the DPPH radical scavenging capacity(P < 0.05). PCA showed that GTR2 scored highest among the eight strains. In conclusion, this strain has a high content of extracellular bioactive ingredients and good antioxidant activity, making it a potential candidate for the development of antioxidant products.更多还原