【摘要】 目的：探究叉分蓼多糖（Polygonum divaricatum L. polysaccharides,PSPDL）体外抗炎活性及其作用机制。方法：从叉分蓼中提取多糖，采用高效凝胶渗透色谱、高效液相色谱、傅里叶变换红外光谱、X射线衍射、核磁共振波谱技术对其结构进行表征，并通过脂多糖诱导RAW264.7细胞炎症模型评估其体外抗炎活性及其作用机制。结果：PSPDL的重均分子质量为59.475 kDa，主要由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成，其物质的量比为1.69∶4.95∶1.04∶21.79∶19.01∶31.68∶19.84，是含有(1→4)-α-D-Glcp的α-吡喃型多糖；能够抑制炎症因子的释放及其相关mRNA的表达，改善氧化应激，下调p38、p-p38、IκB-α、p65和p-p65蛋白的表达水平。结论：PSDPL具有抗炎活性，其作用机制可能与调节炎症相关mRNA表达，调控丝裂原活化蛋白激酶/核转录因子κB信号通路相关，本研究结果可为PSPDL资源的开发与应用提供科学依据。更多还原

【Abstract】 Objective: To explore the in vitro anti-inflammatory activity and action mechanism of a polysaccharide extracted from Polygonum divaricatum L.(PSPDL). Methods: The structure of PSPDL was characterized using high-performance gel permeation chromatography(HPGPC), high-performance liquid chromatography(HPLC), Fourier transform-infrared spectroscopy(FT-IR), X-ray diffraction(XRD), and nuclear magnetic resonance spectroscopy(NMR). The in vitro antiinflammatory activity and action mechanism of PSPDL were investigated in lipopolysaccharide(LPS)-induced RAW264.7cells. Results: PSPDL had a relative molecular mass of 59.475 kDa and consisted mainly of mannose(Man), rhamnose(Rha), glucuronic acid(GlcA), galacturonic acid(GalA), glucose(Glc), galactose(Gal), and arabinose(Ara), with a molar ratio of 1.69:4.95:1.04:21.79:19.01:31.68:19.84. PSPDL was an α-pyran polysaccharide containing(1→4)-α-D-Glcp linkage. PSPDL inhibited the release of inflammatory factors and related gene mRNA expression, ameliorated oxidative stress, down-regulated the protein expression levels of p38, p-p38, IκB-α, p65, and p-p65. Conclusion: PSPDL exhibited anti-inflammatory activity, perhaps by regulating inflammatory mRNA expression and modulating the mitogen-activated protein kinases/nuclear factor kappa B(MAPK/NF-κB) signaling pathway. This finding provides a scientific basis for the development and utilization of PSPDL resources.更多还原