【摘要】 本研究以豆粕为原料，通过米曲霉固态发酵制备可螯合Cu²⁺的豆粕肽(soybean meal peptide-Cu²⁺,SBMP-Cu（II）)，并探究SBMP-Cu（II）的螯合机制及其结构稳定特性。结果显示1～5 k Da SBMP对Cu²⁺具有较强的螯合能力，经阴离子交换和凝胶过滤层析纯化后，其螯合Cu²⁺能力达到（288.47±2.18）mg/g。利用液相色谱-串联质谱分析鉴定出1～5 k Da SBMP中有27个寡肽，其分子质量均小于1 500 Da，且酸性氨基酸（天冬氨酸和谷氨酸）和碱性氨基酸（精氨酸、组氨酸、赖氨酸）在螯合Cu²⁺过程中起关键作用。傅里叶变换红外光谱、X射线衍射、扫描电镜及Zeta电位等分析结果表明SBMP螯合Cu²⁺的主要活性基团为—COOH和—NH₂，且所得SBMP-Cu（II）的结晶度增加，其表面电荷发生中和反应而形成粗糙球形团聚体。此外，SBMP-Cu（II）具有比SBMP更为优异的耐酸、耐热、耐盐性及体外消化稳定性，可作为新型铜补充剂有效提高Cu²⁺在胃肠道环境的生物可及性。本研究结果可为SBMP-Cu（II）补充剂的低成本高效制备和豆粕高价值资源化利用提供理论依据和技术支持。更多还原

【Abstract】 This study aimed to prepare copper-chelating peptides from soybean meal （SBMP-Cu（II）） using Aspergillus oryzae in solid-state fermentation and to investigate the chelation mechanism,structure and stability of SBMP-Cu（II）.The results showed that peptides from soybean meal peptides （SBMP） with molecular masses of 1–5 k Da had a strong chelating capacity toward Cu²⁺,and the Cu²⁺chelating capacity reached up to （288.47±2.18） mg/g after purification by weak anionexchange chromatography and gel filtration chromatography.A total of 27 oligopeptides with molecular masses of less than1 500 Da were identified from the 1–5 k Da SBMP by liquid chromatography-tandem mass spectrometry （LC-MS/MS）,and acidic （aspartic acid and glutamic acid） and basic amino acids （arginine,histidine and lysine） played a key role in the chelation of Cu²⁺.Fourier transform infrared spectroscopy （FTIR）,X-ray diffraction （XRD）,scanning electron microscopy（SEM） and zeta potential analysis showed that the major active groups in SBMP involved in its chelation with Cu²⁺were–COOH and–NH₂,and SBMP-Cu（II） had a higher crystallinity,with the formation of roughly spherical agglomerates caused by the neutralization of charges on the surface.In addition,SBMP-Cu（II） showed stronger resistance to acid,heat and salt as well as higher in vitro digestive stability than SBMP,suggesting that SBMP-Cu（II） has the potential to improve Cu²⁺bioavailability as a new copper supplement.These results provide a theoretical basis and technical support for the low-cost and efficient preparation of SBMP-Cu（II） supplements and the high-value resource utilization of soybean meal.更多还原