【摘要】 目的：分析褪黑素对白细胞介素-17(IL-17)刺激下类风湿关节炎滑膜细胞活性及相关蛋白Rankl、Bcl-2和Bax表达水平的影响。方法：将类风湿关节炎滑膜细胞分为对照组、IL-17组及IL-17+褪黑素组，分别使用CCK8试剂盒以及蛋白质印迹实验检测细胞活性及相关蛋白Rankl、Bcl-2及Bax表达水平。结果：与对照组比较，IL-17组有更高的细胞活性(P<0.01);而IL-17+褪黑素组的细胞活性低于IL-17组(P<0.01)。与对照组比较，IL-17组的Rankl、Bcl-2蛋白表达水平上升，Bax表达水平下降(P<0.01);与IL-17处理组比较，IL-17+褪黑素组的Rankl、Bcl-2表达水平则下降，Bax表达水平上升(P<0.01)。结论：褪黑素能抑制IL-17对类风湿关节炎滑膜细胞的活性作用，同时褪黑素能减弱IL-17对类风湿关节炎滑膜细胞表达Rankl以及抗凋亡作用。更多还原

【Abstract】 Objective:To analyze the effect of melatonin on the activity of synovial cells and the expression of related proteins Rankl, Bcl-2 and Bax in rheumatoid arthritis stimulated by interleukin-17(IL-17).Methods:Rheumatoid arthritis synovial cells were divided into control group, IL-17 group and IL-17+melatonin group.Cell activity and expression levels of related proteins Rankl, Bcl-2 and Bax were detected by CCK8 kit and western blotting.Results:Compared with the control group, the IL-17 group had higher cell activity(P<0.01).The cell activity of IL-17+melatonin group was lower than that of IL-17 group(P<0.01).Compared with the control group, the expression levels of Rankl and Bcl-2 proteins in the IL-17 group increased, while the expression levels of Bax decreased(P<0.01).Compared with the IL-17 treatment group, the expression levels of Rankl and Bcl-2 in the IL-17+melatonin group decreased, and the expression level of Bax increased(P<0.01).Conclusion:Melatonin can inhibit the activity of IL-17 on rheumatoid arthritis synovial cells, and melatonin can reduce the expression of Rankl and anti apoptosis of IL-17 on rheumatoid arthritis synovial cells.更多还原