【摘要】 本研究以稀有鮈鲫(Gobiocypris rarus)为实验对象，探究不同浓度孔雀石绿对稀有鮈鲫胚胎的致畸性和毒性效应。在稀有鮈鲫通过干法人工授精发育4.5 hpf,通过设置1个空白对照组及5个浓度组(1.0、1.5、2.0、2.5和3.0 mg/L),采用水体浸泡暴露的实验方式研究孔雀石绿对胚胎的影响。结果表明孔雀石绿会导致稀有鮈鲫自主运动频次降低、心跳次数降低、孵化率也大大降低。高浓度(2.5、3.0 mg/L)的孔雀石绿对于稀有鮈鲫胚胎孵化有较高致死效应，低浓度(1.0～2.0 mg/L)的孔雀石绿对于稀有鮈鲫胚胎孵化有显著影响，影响稀有鮈鲫的自主运动、心跳和孵化能力，并且稀有鮈鲫在孔雀石绿的暴露下导致畸形症状，如尾部弯曲、心包囊水肿等。更多还原

【Abstract】 In this paper, Gobiocypris rarus was used as the experimental object to explore the teratogenic and toxic effects of malachite green at different concentrations on embryos of G.rarus.Whose was developed for 4.5 hpf by dry artificial insemination, and the effects of malachite green on embryos was studied by water immersion exposure by setting a blank control group and five concentration groups(1.0 mg/L,1.5 mg/L,2.0 mg/L,2.5 mg/L and 3.0 mg/L).The results showed that the frequency of voluntary movements, the number of jumps, and the hatchability of G.rarus would be decreasing after the malachite green entered.The malachite green in high concentration of 2.5 mg/L and 3.0 mg/L has a high lethal effect on the hatching of G.rarus embryos, while that in low concentration of 1.0 mg/L and 2.0 mg/L has a significant effect on the hatching of G.rarus embryos, affecting the voluntary movement, heartbeat, and hatching ability of G.rarus.G.rarus exposed to malachite green was results in malformation symptoms, such as tail curvature, pericardial cyst edema and so on.更多还原