【摘要】 目的：通过脂多糖（Lipopolysaccharide, LPS）诱导小鼠RAW 264.7巨噬细胞炎症模型，探讨了石榴花多糖对细胞的抗炎效果及机制。方法：通过CCK8法检测不同浓度的石榴花多糖对小鼠RAW 264.7细胞活性的影响。LPS刺激建立细胞炎症模型，加入250,125,31.25μg/mL石榴花多糖进行处理，24 h后Griess法检测细胞上清液NO水平，ELISA法检测细胞上清液TNF-α、IL-1β、IL-6、PGE₂水平，RT-qPCR法检测细胞iNOS、COX2、MyD88、NF-κB mRNA表达。结果：石榴花多糖的质量浓度在0.25 mg/mL时，细胞存活率下降，在0.125 mg/mL以下时RAW 264.7细胞活力上升。与模型组相比，石榴花多糖组细胞上清液NO、TNF-α、IL-1β、IL-6、PGE₂水平均降低，RAW 264.7细胞的iNOS、COX2、MyD88、NF-κB mRNA表达降低（P<0.05）。结论：石榴花多糖可以抑制MyD88/NF-κB信号通路，降低NO释放和炎症因子水平，进而减轻LPS诱导的小鼠巨噬细胞炎症反应。更多还原

【Abstract】 Objective: To investigate the anti-inflammatory effect and mechanism of pomegranate flower polysaccharide in RAW 264.7 macrophage inflammation model of mice induced by Lipopolysaccharide（LPS）.Methods: The effects of different concentrations of pomegranate flower polysaccharide on the activity of RAW 264.7 cells were detected by CCK8 assay.The cell inflammation model was established after LPS stimulation, and 250,125,31.25 μg/mL pomegranate polysaccharide was added for treatment.After 24 h, NO levels in cell supernatant were detected by Griess method, and TNF-α,IL-1β,IL-6 and PGE₂ levels in cell supernatant were detected by ELISA method.The mRNA expressions of iNOS,COX2,MyD88 and NF-κB were detected by RT-qPCR.Results: When the mass concentration of pomegranate flower polysaccharide was 0.25 mg/mL,the cell survival rate decreased, and when the mass concentration was below 0.125 mg/mL,the cell viability of RAW 264.7 increased.Compared with model group, the levels of NO,TNF-α,IL-1β,IL-6 and PGE₂ in cell supernatant of pomegranate flower polysaccharide group were decreased, and the mRNA expressions of iNOS,COX2,MyD88 and NF-κB in RAW 264.7 cells were decreased（P<0.05）.Conclusion: Pomegranate flower polysaccharide can inhibit MyD88/NF-κB signaling pathway, reduce the level of NO release and inflammatory factors, and then reduce the inflammatory response of mouse macrophages induced by LPS.更多还原