【摘要】 本文对无糖可乐中的甜蜜素含量测定方法进行研究，优化了色谱分离条件，建立了离子交换-电导法测定无糖可乐中的甜蜜素的方法。该方法使用的色谱柱是Thermo IonPac AG11-HC(50mm×4mm)保护柱以及Thermo IonPac AS11-HC(250mm×4mm)分离柱，KOH梯度淋洗，抑制电导检测。结果表明，甜蜜素在0.112mg/L～112mg/L时有良好的线性关系，相关系数在0.999以上；方法检出限为0.05mg/L，系统相对偏差在2%以内，回收率98.22%～103.9%。该方法操作简便，分析快速，重现性好，结果准确、可靠，适用于无糖可乐中甜蜜素的含量测定。更多还原

【Abstract】 The study reported the establishment of an ion chromatography method to determine sodium cyclamate in diet coke by ion chromatography using conductivity detector. The separation was achieved on a Thermo IonPac AG11-HC guard column and a Thermo IonPac AS11-HC analytical column with KOH as the gradient eluent and suppressed conductivity detector. Results showed that sodium cyclamate had good liner relations when the concentration was in the range of 0.112mg/L～112mg/L and the correlation coefficient was above 0.999. The detection limit was 0.05mg/L.The system relative deviation was less than 2%,and the recovery for diet coke was in the range of 98.22%～103.9%.The method is simple,rapid,sensitive,accurate and reproducible. It was suitable for determination of sodium cyclamate in diet coke.更多还原