【摘要】 目的：基于16S rDNA测序探讨早发性卵巢功能不全（POI）肾虚肝郁证患者肠道菌群的群落结构变化。方法：纳入POI肾虚肝郁证患者60例（POI组），招募健康志愿者30例（健康对照组）。通过16S rDNA高通量测序分析POI肾虚肝郁证患者及健康志愿者的肠道菌群特征，并进行对比分析。结果：与健康对照组比较，在门水平上POI组拟杆菌门等相对丰度升高，而厚壁菌门、放线菌门等相对丰度降低，在属水平上POI组普雷沃氏菌属、拟杆菌属和梭状芽胞杆菌属XIVa等相对丰度升高，而粪杆菌属等相对丰度降低。结论：POI患者与健康人群在肠道菌群上存在差异，肠道菌群之间的比例失调可能引起机体内环境紊乱，诱导POI发生。更多还原

【Abstract】 Objective:To explore and analyze the changes in community structure of intestinal flora in patients with premature ovarian insufficiency(POI) with kidney deficiency and liver constraint syndrome based on 16S rDNA sequencing. Methods:A total of 60 cases of POI patients with kidney deficiency and liver constraint syndrome were included(POI group), and 30 healthy volunteers were recruited(healthy control group). The characteristics of intestinal flora of POI patients with kidney deficiency and liver constraint syndrome and healthy volunteers were analyzed by high-throughput 16S rDNA sequencing,and comparative was conducted. Results:Compared with those in the healthy control group,at the phylum level,the relative abundance of bacteroides was increased in the POI group,and the relative abundance of firmicutes and actinomycetes was decreased; at the genus level, the relative abundance of provetella,bacteroides, and clostridium XIVa was increased in the POI group, and the relative abundance of fecal bacillus was decreased. Conclusion: There are differences in intestinal flora between POI patients and healthy groups,and the imbalance in the proportion of intestinal flora may cause disturbances in the internal environment of the organism,leading to the occurrence of POI.更多还原