【摘要】 目的 分析乳腺癌组织及癌旁正常乳腺组织Claudin6、MMP2及Snail的蛋白水平，三者在乳腺癌组织中表达的相关性，上述三种蛋白表达与患者病理分期、淋巴结转移、肿瘤大小和年龄的相关性。方法 选取2021年8月—2022年7月本院病理中心的38例乳腺癌和24例癌旁正常乳腺组织标本，采用免疫组化Sp法进行实验研究，检测Claudin6、MMP2及Snail的表达情况；分析乳腺癌组织上述三种蛋白表达及其与临床病理特征的相关性。结果 癌组织中Claudin6阳性率较正常组织低，差异显著(P<0.01);癌组织中MMP2和Snail阳性表达率较正常组织高，差异具有统计学意义(P<0.05)。癌组织中Claudin6与MMP2表达呈负相关(P<0.01),Claudin6与Snai表达呈负相关(P<0.001),MMP2与Snail表达呈正相关(P≤0.001)。Claudin6、MMP2和Snail的表达水平与病理分期、肿瘤大小和患者年龄均不相关(P>0.05);淋巴结转移病例Claudin6阳性率显著低于无淋巴结转移病例(P<0.01);相反，淋巴结转移病例MMP2和Snail阳性率明显高于无淋巴结转移病例(P<0.05)。结论 Claudin6蛋白在乳腺癌组织中低表达、MMp2及Snail蛋白在乳腺癌组织中高表达，在乳腺癌组织中Claudin6与MMP2蛋白、Claudin6与Snail蛋白表达均呈负相关；MMP2和Snail蛋白表达呈正相关。淋巴结转移病例CLDN6阳性率显著低于无淋巴结转移病例；淋巴结转移病例MMP2和Snail阳性率高于无淋巴结转移病例；推测紧密连接蛋白6可能通过阻断Snail/MMP2信号轴，抑制乳腺癌的上皮间质转化和转移。更多还原

【Abstract】 Objective To analyse the expression level of Claudin6(CLDN6), Matrix metalloproteinases(2MMP2) and Snail in breast cancer tissues and adjacent normal breast tissues, and to investigate the correlation among the expression of the three proteins and the correlation between the their expressions and the pathological stage, lymphatic metastasis, tumor size, and age.Methods 38 breast cancer tissues and 24 adjacent normal breast tissues which were sampled in the pathology center of our university during August 2021 and July 2022 were selected as study samples. Immunohistochemical Sp method was used in our experiment to detect the expressions of Claudin6, MMP2 and Snail proteins. Then the correlations between the proteins and clinicopathologic feature were analyzed. Results The positive rate of Claudin6 in cancer tissues was significantly lower than that in normal tissues(P<0.01). The positive rates of MMP2 and Snail in cancer tissues were significantly higher than those in normal tissues(P<0.05). The expression of Claudin6 was negatively correlated with MMP2 in cancer tissues(P<0.01), and the expression of Claudin6 was negatively correlated with Snail(P<0.001), while MMP2 was positively correlated with Snail(P≤0.001). The levels of Claudin6, MMP2 and Snail were not correlated with pathological stage, tumor size and age(P>0.05). The positive rate of Claudin6 in patients with lymph node metastasis was significantly lower than that without lymph node metastasis(P<0.01). On the contrary, the positive rates of MMP2 and Snail in patients with lymph node metastasis were significantly higher than those without lymph node metastasis(P<0.05).Conclusions Claudin6 is low expressed in breast cancer tissues, while MMP2 and Snail are expressed at a high level in breast cancer tissues.There is a negative correlation between the expression of Claudin6 and MMP2 and Snail, while MMP2 is positively correlated with Snail. The positive rate of Claudin6 in patients with lymph node metastasis is significantly lower than that without lymph node metastasis. On the contrary, MMP2 and Snail are higher in patients with lymph node metastasis. We speculate that Claudin6 may inhibit EMT and metastasis by blocking the Snail/MMP2 signaling axis in breast cancer.更多还原