【摘要】 建立并优化了测定羟丙基壳聚糖(HPCS)摩尔取代度(M_S)的气相色谱法(GC法),并与~1H NMR法进行比较。结果表明，用特氟龙内衬-铝盖的反应瓶进行样品前处理较合理，自动进样可提高精密度，标准曲线测定法优于美国药典中的单点法；所测M_S值与~1H NMR法的结果呈线性关系，其校正系数为1.85;当HPCS的原料壳聚糖的乙酰化度(DA)小于5%时，对M_S的测定的影响较小，其相对误差<1%。因此，可成为HPCS生产企业的常规检测方法，用于大批量测试。更多还原

【Abstract】 In this paper, a gas chromatography method(GC method) for the determination of molar substitution degree(M_S) of hydroxypropyl chitosan(HPCS) was established and optimized, and compared with ~1H NMR method. The results show that it is reasonable to use reaction vials with teflon-lined and aluminum-cap for sample pretreatment, automatic sampling improves precision. The standard curve method is superior to the single-point method in the US Pharmacopoeia. The measured M_S values have a linear relationship with those from ~1H NMR method, and its correction coefficient is 1.85. When the acetylation degree(DA) of the raw material chitosan is less than 5%, little effect on the M_S measurement, and the relative error is less than 1%. Therefore, this method can be used as a routine test in HPCS manufacturers for large-scale of measurement.更多还原