【摘要】 目的：探讨TMSB10 mRNA在原发性肝癌中的表达及其与免疫浸润和预后的关系。方法：从TCGA数据库中下载原发性肝癌数据集，分析TMSB10 mRNA在原发性肝癌中的表达及其与生存率、免疫细胞浸润的相关性。纳入我院150例原发性肝癌患者癌组织及癌旁组织，免疫组化法分析TMSB10mRNA在癌组织及癌旁组织中表达水平的差异。原发性肝癌患者根据随访结果分为预后不良组（n=81）和预后良好组（n=69），分析原发性肝癌预后不良的危险因素。结果：原发性肝癌组织中TMSB10mRNA的表达水平较癌旁组织显著增加（P<0.001）。原发性肝癌患者TMSB10 mRNA低表达组和高表达组的中位生存时间为84.4月和49.7月，与低表达组相比，高表达组生存率显著降低（HR=1.55,P=0.039）。原发性肝癌组织中TMSB10 mRNA表达水平与B淋巴细胞、CD8+T淋巴细胞、CD4+T淋巴细胞、巨噬细胞、中性粒细胞和树突状细胞浸润程度均呈正相关。原发性肝癌组织中TMSB10高表达率显著高于癌旁组织（χ~2=103.419,P<0.001）。血管侵犯、AJCC分期为Ⅲ～Ⅳ期和TMSB高表达是原发性肝癌患者预后不良的独立危险因素。预后不良患者中，TMSB10高表达患者复发、转移时间较低表达患者显著缩短（t=4.456,P<0.001）。结论：TMSB10 mRNA在原发性肝癌组织中高表达，其表达水平与免疫浸润呈正相关，是患者预后不良的生物学标志物之一。更多还原

【Abstract】 Objective: To investigate the expression of TMSB10 mRNA in primary liver cancer and its clinical value. Methods: The primary liver cancer data set was downloaded from the TCGA database and to analyze the expression of TMSB10 mRNA in primary liver cancer. The association between TMSB10 mRNA expression and primary liver cancer patients survival rate, immune cell infiltration were analyzed. A total of 150 primary liver cancer tissues and adjacent tissues were enrolled in our hospital, and the expression of TMSB10 mRNA in primary liver cancer tissues and adjacent tissues were analyzed via immunohistochemistry. All patients with primary liver cancer were followed up and divide into poor prognosis(n=81) and good prognosis group(n=69). The Logistic regression analysis was used to analyze the risk factors for poor prognosis in patients with primary liver cancer. Results: The expression of TMSB10 mRNA in primary liver cancer tissue and normal liver tissue were 1111.239(601.842, 2154.575), 396.998(324.379, 524.741),respectively. When compared with normal liver tissue, the TMSB10 mRNA was significantly overexpressed in primary liver cancer tissue(P<0.001). The median survival time of the TMSB10mRNA lower expression group was 84.4 months, while the median survival time was 49.7 months in TMSB10 mRNA higher expression group. Compared with the TMSB10 lower expression group,the survival rate of the TMSB10 higher expression group was significantly decreased(HR=1.55, P=0.039). The expression of TMSB10 mRNA in primary liver cancer tissue was significantly positively correlated with the infiltration degree of B lymphocytes, CD8+T lymphocytes, CD4+T lymphocytes, macrophages, neutrophils, and dendritic cells. The high expression rate of TMSB10 in primary liver cancer tissues was significantly higher than that in adjacent tissues(χ~2=103.419, P<0.001). The Logistic analysis showed that vascular invasion, AJCC stage with III-IV, and higher expression of TMSB were all independent risk factors for the poor prognosis in patients with primary liver cancer. In poor prognosis group, when compared with TMSB10 lower expression group, the time of recurrence and metastasis in TMSB10 higher expression group was significantly shortened(t=4.456, P<0.001). Conclusion: TMSB10 was significantly over-expressed in primary liver cancer, and TMSB can serve as a biological marker for poor prognosis in patients with primary liver cancer.更多还原