【摘要】 目的：探究miR-133a是否可通过调节G6PD参与肝细胞癌（HCC）的发生与发展进程。方法：生物信息学分析预测miR-133a和G6PD的结合位点；RT-PCR或Western blot检测miR-133a和G6PD在HCC组织及癌旁组织中的表达；CCK-8、流式细胞术实验检测miR-133a/G6PD对HCC细胞生长、凋亡的影响；荧光报告基因实验及Western blot技术检测miR-133a对G6PD表达的影响。结果：在HCC组织中miR-133a低表达，而G6PD高表达（P<0.01）；过表达miR-133a降低了G6PD的表达（P<0.01）；过表达miR-133a明显抑制了Hep G2细胞增殖，并促进了细胞凋亡（P<0.05）。结论：miR-133a通过降低G6PD的表达抑制HCC的发生及发展。更多还原

【Abstract】 Objective: To explore if miR-133a is involved in the occurrence and development of hepatocellular carcinoma(HCC) via regulating G6PD. Methods: Bioinformatics analysis predicted the binding sites of miR-133a and G6PD; RT-PCR or western blot was used to assess the expression of miR-133a and G6PD in HCC tissues and the adjacent normal tissues; CCK-8 and flow cytometry assays were performed to evaluate the effects of miR-133a/G6PD on cell proliferation, apoptosis; Fluorescent reporter gene and western blot assays were used to assess the effect of miR-133a on G6PD expression. Results: miR-133a expression was decreased in HCC tissues while G6PD was increased(P0.01); Up-regulation of miR-133a significantly reduced G6PD expression(P<0.01); up-regulation of miR-133a inhibited cell growth and promoted cell apoptosis(P<0.05), whereas these effects induced by miR-133a over-expression were all abolished when G6PD was up-regulated(P<0.01).Conclusion: miR-133a represses the occurrence and development of HCC via targeting G6PD.更多还原