【摘要】 目的：探讨胃癌中胶质瘤相关癌基因1(GLI1)的表达与免疫浸润相关性，及其与临床预后的相关性。研究GLI1的表达对胃癌化疗药物耐药性的影响。方法：运用TCGA数据库分析GLI1在胃癌和正常组织中的表达差异，分析胃癌患者临床特征和GLI1基因表达水平对患者预后的影响；分析胃癌组织中GLI1基因表达与肿瘤免疫细胞浸润的相关性，探究其对化疗药物及靶向药物耐药性的影响。收集临床标本，分析GLI1在胃癌和癌旁组织中的表达差异。结果：胃癌组织中GLI1高表达是正常组织的1.7倍，其高表达患者总生存期、无病生存期较低表达患者更短（P<0.05）。胃癌组织中GLI1的高表达组间质评分、免疫评分、免疫浸润细胞丰度更高，GLI1高表达对药物敏感性降低并且和免疫检查点标志物PDCD1表达呈正相关（P<0.05）。GLI1在低分化胃癌患者中表达明显升高（P<0.05）。结论：GLI1表达与胃癌患者预后及免疫浸润相关，并且其可能通过调节化疗耐药而导致患者的不良预后，可能是胃癌潜在的治疗靶点和分子标志物。更多还原

【Abstract】 Objective: To investigate the correlation between the expression of GLI1 and immune invasion and clinical prognosis in gastric cancer. To study the effect of GLI1 expression on drug resistance in gastric cancer. Methods: The expression difference of GLI1 in gastric cancer and normal tissues was analyzed by using TCGA database, and the effect of clinical features and GLI1 gene expression level on prognosis of patients with gastric cancer was analyzed. The correlation between GLI1gene expression and tumor immune cell infiltration in gastric cancer tissues was analyzed to explore its influence on drug resistance of chemotherapy drugs and targeted drugs. Clinical samples were collected to analyze the difference of GLI1 expression in gastric cancer and paracancer tissues. Results: The expression of GLI1 in gastric cancer tissues was 1.7 times that in normal tissues, and the overall survival and disease-free survival of patients with high expression are shorter than those with low expression(P < 0.05). The interstitial score, immune score and abundance of immunoinfiltrating cells were higher in the high expression of GLI1 in gastric cancer tissues. High expression of GLI1 reduces drug sensitivity and is positively correlated with the expression of immune checkpoint markers PDCD1(P <0.05). GLI1 expression was significantly increased in patients with subdifferentiated gastric cancer.Conclusions: GLI1 expression is associated with the prognosis and immune infiltration of patients with gastric cancer, and it may lead to poor prognosis of patients by regulating chemotherapy resistance, which may be a potential therapeutic target and molecular marker for gastric cancer.更多还原