【摘要】 建立基于氨基化-多壁碳纳米管(amino-functionalized multi-walled carbon nanotubes,NH2-MWCNTs)改进的QuEChERS净化方法，结合液相色谱-串联质谱(LC-MS/MS)测定3种热带水果中110种农药及其代谢物残留量。样品经乙腈提取，采用欧洲标准(EN. 15662)萃取缓冲体系QuEChERS盐包提取，NH2-MWCNTs改良的分散固相萃取(d-SPE)净化管净化，多反应监测模式(MRM)分析，基质匹配标准曲线外标法定量。结果表明：在0.002～0.2 mg/L范围内110种农药及其代谢物具有良好的线性关系，决定系数(R2)均高于0.9902，定量限(LOQ)为0.005～0.05 mg/kg，添加回收率为61%～120%，相对标准偏差(RSD)为0.30%～15%(n=6)。该方法净化效果好，能在短时间内可靠地分析样品。灵敏度、正确度、精密度均满足日常检测定性、定量需求，适用于荔枝、龙眼和香蕉等热带水果中农药多残留的快速检测。更多还原

【Abstract】 Combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS), an improved QuEChERS method was established based on amino-functionalized multi-walled carbon nanotubes(NH2-MWCNTs) for the determination of 110 pesticides and their metabolites in three tropical fruits. The samples were extracted by acetonitrile, salted out by QuEChERS salt package with European standard(EN.15662) buffer system, purified by dispersed solid phase extraction(d-SPE) with NH2-MWCNTs sorbents, analyzed at multi-reaction monitoring mode(MRM), and quantified using external standard method with matrix-matched calibration curves. The results showed good linearity(R2 >0.9902) for the 110 pesticides and their metabolites in the range of 0.002-0.2 mg/L. The limits of quantification(LOQs) ranged from 0.005 to 0.05 mg/kg. The recoveries were 61%～120%, and the relative standard deviations(RSDs) were 0.30% to 15%(n=6). This method exhibits excellent purification performance and takes less time for the sample analysis. The validation results met the requirements for qualitative and quantitative analysis of pesticides with high sensitivity, accuracy, and precision. It is suitable for rapid detection of multiple pesticide residues in tropical fruits such as litchi,longan and banana.更多还原