【摘要】 N~6-甲基腺嘌呤(N~6-methyladenosine,m~6A)修饰是真核生物中最常见的RNA修饰。包含YT521-B同源(YT521-B homology,YTH)结构域的蛋白可识别m~6A修饰，从而介导RNA代谢。该类蛋白在植物病原真菌中的研究还未见报道。本研究利用生物信息学方法，对127种植物病原真菌中的m~6A识别蛋白进行了系统鉴定和分析，并对该类蛋白在玉米大斑病菌(Setosphaeria turcica)发育过程中的基因表达模式进行分析。结果发现，在供试的127个菌株中共鉴定了225个m~6A识别蛋白，其中担子菌门中93%真菌含有2个m~6A识别蛋白；理化性质分析发现，在鉴定的225个m~6A识别蛋白中，有67.56%的蛋白呈碱性，且不同门及不同生活方式真菌的m~6A识别蛋白酸碱性存在显著差异。系统发育分析发现，225个m~6A识别蛋白可分为5组，其中2组与人类(Homo sapiens)具有较近的进化关系，相同门和相同生活方式真菌的m~6A识别蛋白倾向于聚类在同一进化分支上；保守位点分析发现，YTH结构域序列在所有鉴定的m~6A识别蛋白中存在多个保守型位点。在玉米大斑病菌中鉴定了1个编码该类蛋白的基因St YTH1，表达量分析发现，其在病菌附着胞发育时期的表达量最高，可能对病菌附着胞发育具有重要的调控作用。本研究为深入揭示m~6A识别蛋白的功能提供了基础资料。更多还原

【Abstract】 The N~6-methyladenosine (m~6A) is the most common RNA modification in eukaryotes.Proteins containing the YT521-B (YTH) domain recognize the m~6A site and mediate RNA metabolism.However,the function of m~6A recognition proteins in plant pathogenic fungi has not been reported yet.This study systematically identified and analyzed the m~6A recognition proteins in 127 plant pathogenic fungi using bioinformatics method.Furthermore,the expression patterns of the genes encoding m~6A recognition proteins during the development of Setosphaeria turcica were analyzed.The results showed that a total of 225 m~6A recognition proteins were identified in the tested 127 fungi,and 93%of the fungi in the Basidiomycota phylum had 2 m~6A recognition proteins.Physicochemical analysis showed that 67.56%of the m~6A recognized proteins identified were alkaline.Notably,significant differences in the acidity and alkalinity of these proteins were observed within different phyla and fungal lifestyles.Phylogenetic analysis revealed that 225 m~6A recognition proteins could be classified into 5 groups,2 of which shared a closer evolutionary relationship with humans(Homo sapiens).Additionally,m~6A recognition proteins from fungi with the same phylum and lifestyle tended to cluster together on evolutionary branches.The conserved site analysis uncovered the existence of several conserved-type sites in the sequences of the YTH domain across the 225 m~6A recognized proteins.A gene that encodes the m~6A recognition protein,namely St YTH1,was identified in S.turcica.Expression analysis revealed that St YTH1 had the highest expression level in the developmental stage of appressorium,suggesting that St YTH1 might be involved in regulating the development of appressorium.The present study provides basic materials for an insightful revelation of the functions of the m~6A recognition proteins.更多还原