【摘要】 目的：研究有氧运动、抗阻运动、有氧联合抗阻运动3种不同方式运动对胰岛素抵抗(IR)大鼠骨骼肌游离脂肪酸(FFA)及腺苷酸活化蛋白激酶(AMPK)、乙酰辅酶A羧化酶(ACC)、肉毒碱棕榈酰基转移酶(CPT1)活性水平的影响，为探讨不同方式运动对胰岛素抵抗大鼠骨骼肌脂肪沉积的效果及AMPK-ACC途径在骨骼肌中调节脂肪酸氧化的作用。方法：高糖高脂饲料喂养建立胰岛素抵抗大鼠模型之后改为普食饲养，并随机分为高脂改善食对照组(C组)、有氧运动组(E组)、抗阻运动组(R组)、有氧+抗阻联合运动组(RE组),运动干预结束后检测股四头肌FFA、AMPK、ACC、CPT1酶活性水平。结果：(1)13周高糖高脂饮食造模后，与P组相比，造模组C组口服葡萄糖耐量、胰岛素耐量在30 min、60 min、曲线下面积AUC均有极显著性差异(P<0.01),造模组C组大鼠FBG无显著性差异(P>0.05);造模组C组FINS、ISI、HOMA-IR较P组均有极显著性差异(P<0.01)。(2)与C组相比，E组、R组、RE组血清FFA无显著差异(P>0.05);股四头肌FFA均显著降低(P<0.01),但各运动组间无显著差异(P>0.05)。(3)与C组相比，E组、R组、RE组AMPK酶活性均显著升高(P<0.01),RE组AMPK酶活性较E组、R组均显著升高(P<0.01);与C组相比，E组、RE组ACC酶活性有降低趋势，而R组则升高，但均无显著性差异(P>0.05),各运动组之间ACC酶活性也未见差异(P>0.05);各运动组CPT1酶活性较C组均显著升高(P<0.01),RE组CPT1酶活性较E组、R组均显著升高(P<0.01)。结论：(1)13周高糖高脂饮食干预成功造成胰岛素抵抗大鼠模型。(2)8周期不同运动均能显著降低IR大鼠骨骼肌脂肪沉积，其机制可能与运动显著上调IR大鼠骨骼肌AMPK、CPT1酶活性水平，增强脂肪氧化利用，改善胰岛素抵抗有关；联合有氧与抗阻运动较单一运动对脂肪氧化效果更为显著。(3)8周期不同方式运动干预未观察到IR大鼠ACC、CPT1的同步变化。IR大鼠骨骼肌脂肪酸氧化的调节可能独立于AMPK/ACC途径，ACC酶活性的调节对于降低脂肪含量是否重要，尚需进一步研究。更多还原

【Abstract】 Objective: To study the effects of aerobic exercise, resistance exercise and aerobic combined resistance exercise on the activities of free fatty acid(FFA) and adenylate activated protein kinase(AMPK), acetyl coenzyme A carboxylase(ACC) and carnitine palmitoyl transferase(CPT1) in skeletal muscle of insulin resistant rats, to investigate the effects of different exercise modes on fat deposition in skeletal muscle of insulin resistant rats and the role of AMPK-ACC pathway in regulating fatty acid oxidation in skeletal muscle.Methods: Rats fed with high-sugar and high-fat diet were randomly divided into control group(group C),aerobic exercise group(group E),resistance exercise group(group R),aerobic + resistance combined exercise group(group RE).FFA,AMPK,ACC and CPT1 activities of quadriceps femoris were measured after exercise intervention.Results:(1)Compared with group P,the oral glucose tolerance, insulin tolerance in group C were significantly different(P<0.01)after 13 weeks of high-sugar and high-fat diet, and the area under curve of AUC in group C was significantly different(P<0.01). There was no significant difference in FBG in group C(P>0.05);FINS,ISI and HOMA-IR in group C were significantly different from those in group P(P<0.01).(2)Compared with group C,FFA in serum of group E,group R and group RE had no significant difference(P> 0.05);FFA in quadriceps femoris was significantly decreased(P<0.01), but there was no significant difference among exercise groups(P>0.05).(3) Compared with group C,the activity of AMPK in group E,R and RE increased significantly(P<0.01),and the activity of AMPK in group RE increased significantly(P<0.01); compared with group E and group R,the activity of ACC in group E and group RE decreased, while that in group R increased, but there was no significant difference(P>0.05), and there was no difference in the activity of CPT1 between exercise groups(P>0.05).The activity of CPT1 in RE group was significantly higher than that in E group and R group(P<0.01).Conclusion:(1)The insulin resistance rat model was successfully established after 13 weeks of high-sugar and high-fat diet intervention.(2)Eight cycles of exercise can significantly reduce the fat deposition in skeletal muscle of IR rats.The mechanism may be that exercise can significantly increase the activities of AMPK and CPT1 in skeletal muscle of IR rats, enhance the utilization of lipid oxidation and improve insulin resistance; combined aerobic and resistance exercise has more significant effect on fat oxidation than single exercise.(3) Synchronized changes of ACC and CPT1 were not observed in IR rats after 8-cycle exercise intervention in different ways.The regulation of fatty acid oxidation in skeletal muscle of IR rats may be independent of AMPK/ACC pathway. whether the regulation of ACC activity is important for reducing fat content needs further study.更多还原