【摘要】 【目的】明确羊肚菌种质资源间的亲缘关系和多样性，为选育适宜内蒙古西部地区栽培的新品种提供参考。【方法】以A3、赵二六、羊贵妃、金秋、DY008、A芦、001、内石林8个羊肚菌菌株为试验材料，利用ISSR分子标记技术对8个羊肚菌菌株进行聚类分析和遗传多样性比较。【结果】利用100条ISSR引物对8个羊肚菌菌株进行PCR检测，筛选出12条ISSR引物，扩增出DNA条带76条。其中，有效条带59条，多态性比率为75.83%。基因多样性指数（H）为0.206 6,Shannon′s信息指数（Ⅰ）为0.330 0。供试菌株的遗传距离变幅最大的为1.072 6；在GS值约为0.38时，将菌株分为5个类群。【结论】供试羊肚菌菌株之间具有明显的遗传差异性。更多还原

【Abstract】 【Objective】To clarify the phylogenetic relationship and diversity of Morchella esculents germplasm resources,and to provide reference for the selection and breeding of new varieties adapted to the cultivation in the western region of Inner Mongolia.【Methods】 8 Morchella esculents strains(A3,Zhao′erliu,Yangguifei,Jinqiu,DY008,A Lu,001 and Neishilin)were used as experimental materials,ISSR molecular marker technology was used to perform cluster analysis and genetic diversity comparison on 8 strains of Morchella esculents. 【Results】Eight Morchella esculents strains were detected by PCR using 100 ISSR primers,12 ISSR primers were selected,and 76 DNA bands were amplified. There were 59 effective bands,and the polymorphism ratio was 75.83%. The gene diversity index(H) was 0.206 6,and Shannon′s information index(Ⅰ) was 0.330 0.The maximal variation of genetic distance was 1.072 6. When the GS value was about 0.38,the strains were divided into5 groups.【Conclusion】 There was significant genetic variability among the tested Morchella esculents strains.更多还原