【摘要】 目的:建立星夏止痛贴膏中生川乌、生草乌所含双酯型生物碱成分含量的HPLC同时测定方法。方法:采用Phenomenex Luna Omega PS C₁₈（4.6 mm×250 mm, 5μm）色谱柱分离，乙腈-四氢呋喃（25∶15）-0.1 moL/L醋酸铵为流动相梯度洗脱，检测波长：235 nm;柱温：25℃;流速：1.0 mL/min,外标法定量。结果:3种双酯型生物碱成分分离度良好，分别在14.151～113.207μg/mL、13.392～107.136μg/mL、14.196～113.564μg/mL浓度范围内与峰面积呈良好的线性关系，平均回收率分别为94.03%、97.92%、94.45%,RSD分别为5.51%、7.09%、6.94%。结论:该方法简便、快速、准确，可同时对星夏止痛贴膏中的乌头碱、次乌头碱、新乌头碱3双酯型生物碱进行分析，能有效地消除杂质干扰，为星夏止痛贴膏的质量评价提供实验依据。更多还原

【Abstract】 Objective A high performance liquid chromatography（HPLC）was established for the simultaneous determination of 3 diester diterpenoid alkaloids, i.e. mesaconitine, aconitine and hypaconitine in Xingxia Zhitong plaster. Methods The 3 alkaloids were separated on Phenomenex Luna Omega PS C₁₈（4.6 mm×250 mm, 5 μm）column with acetonitrile-tetrahydrofuran（25∶15）-ammonium acetate as the mobile phase by gradient elution at a flow rate of 1.0 mL·min^-1. The detective wavelength was235 nm, the column temperature was 25 ℃,and finally quantified by external standard calibration method.Results The 3 alkaloids showed a good linearity relationship between 14.151-113.207 μg/mL、13.392-107.136 μg/mL、14.196-113.564 μg/mL,and the average recoveries were 94.03%（RSD=5.51%）、97.92%（RSD=7.09%）、94.45%（RSD=6.94%）, respectively.Conclusion The method is rapid, simple and accurate, it can simultaneously analyze the contents of 3 alkaloids in Xingxia Zhitong plaster and eliminate the impurity interference effectively. This method and test data also provided reference for the quality evaluation of Xingxia Zhitong plaster.更多还原