【摘要】 目的:建立参苓白术散中苍术酮、白术内酯Ⅰ和甘草苷的定量检测方法。方法:采用HPLC法，以C₁₈色谱柱（5μm, 4.6 mm×250 mm）,乙腈作为流动相A,测定了参苓白术散中苍术酮、白术内酯Ⅰ、甘草苷的含量。结果:苍术酮在0.0082～0.1308 mg/mL浓度范围内线性关系良好，平均加样回收率97.8%（RSD=0.7%,n=6）;白术内酯Ⅰ在0.0016～0.0256 mg/mL浓度范围内线性关系良好，平均加样回收率103.4%（RSD=1.8%,n=6）;甘草苷在0.0153～0.2444 mg/mL浓度范围内线性关系良好，平均加样回收率104.2%（RSD=0.5%,n=6）。结论:本研究成功建立了参苓白术散中苍术酮、白术内酯I和甘草苷的检测方法，且方法专属性强，精密度、重复性良好。更多还原

【Abstract】 Objective A method for the determination of Atractyloketone, Atractylolactone Ⅰ and Liquiritin in Shenling Baizhu Powder was established.Methods The contents of Atractylone, Atractylide Ⅰ and Liquiritin in Shenling Baizhu Powder were determined by HPLC on C₁₈ column（5 μm, 4.6×250 mm） with acetonitrile as mobile phase A.Results Atractylone had a good linear relationship in the concentration range of 0.0082-0.1308 mg/mL, and the average sample recovery rate was 97.8%（RSD=0.7%, n=6）; Atractylide Ⅰ had a good linear relationship in the concentration range of 0.0016-0.0256 mg/mL, and the average sample recovery rate was 103.4%（RSD=1.8%, n=6）; The linear relationship of Liquiritin in the concentration range of 0.0153-0.2444 mg/mL was good, and the average sample recovery rate was104.2%（RSD=0.5%,n=6）.Conclusion In this study, a method for the detection of Atractyloketone, Atractylolactone Ⅰ and Liquiritin in Shenling Baizhu Powder was established successfully, and the method had strong specificity, good precision and repeatability.更多还原