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地黄对斜纹夜蛾生长发育的影响

Effects of Rehmannia glutinosa on the growth and development of Spodoptera litura

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【作者】 梁怀方王丽朱香镇张开心李东阳崔金杰雒珺瑜陈招荣

【Author】 Liang Huaifang;Wang Li;Zhu Xiangzhen;Zhang Kaixin;Li Dongyang;Cui Jinjie;Luo Junyu;Chen Zhaorong;Institute of Cotton Research of Chinese Academy of Agricultural Sciences/National Key Laboratory of Cotton Bio-breeding and Integrated Utilization;Tianjin Agricultural University;

【通讯作者】 雒珺瑜;陈招荣;

【机构】 中国农业科学院棉花研究所/棉花生物育种与综合利用全国重点实验室天津农学院

【摘要】 【目的】斜纹夜蛾是危害棉田的重要害虫,地黄是农田常见杂草,探索并利用地黄防治斜纹夜蛾对于棉花可持续生产具有重要意义。【方法】分别将地黄根或叶干粉混拌于斜纹夜蛾人工饲料(地黄干粉与饲料的质量比分别为1∶3、1∶6、1∶9和1∶18),初步明确地黄对斜纹夜蛾幼虫的死亡率、发育历期和体重的影响;进一步采用药膜法,研究地黄根或叶干粉的95%乙醇提取液(地黄干粉与提取溶剂的料液比分别为1∶50、1∶30和1∶10)对1~6龄斜纹夜蛾幼虫死亡率的影响。【结果】取食混有地黄根或叶干粉饲料的斜纹夜蛾幼虫生长发育受到一定的影响,随着饲料中地黄根或叶干粉含量的增大,斜纹夜蛾幼虫死亡率升高、发育历期延长、体重降低。地黄根或叶干粉与饲料的质量比为1∶3时,其对斜纹夜蛾幼虫的抑制效果最好。地黄根或叶提取液对低龄斜纹夜蛾幼虫有一定抑制作用,对高龄幼虫的毒杀作用较差,同一测定时间随地黄提取液浓度增大,斜纹夜蛾低龄幼虫的死亡率升高。地黄根或叶干粉与提取溶剂的料液比为1∶10时,对斜纹夜蛾1~6龄幼虫的毒杀作用效果最好。【结论】地黄对斜纹夜蛾有一定的抑制作用,在一定范围内,用量越大其抑制作用越强,研究结果可为有效利用农田杂草资源开发植物源杀虫剂奠定理论基础。

【Abstract】 [Objective] Spodoptera litura significantly impacts cotton cultivation, while Rehmannia glutinosa is a prevalent agricultural weed. The exploration and application of R. glutinosa for controlling the S. litura hold crucial relevance for the sustainable production of cotton. [Methods] The effects of root or leaf dry powder of R. glutinosa on the mortality,developmental duration, and body weight of S. litura larvae were preliminarily determined by mixing the dry powder and feed with the mass ratios of 1∶3, 1∶6, 1∶9, and 1∶18, respectively. Additionally, the influence of 95% ethanol extracts of root or leaf powder of R. glutinosa(the ratios of dry powder to solvent were 1∶50, 1∶30, and 1∶10) on the mortality of 1st to 6th instar S. litura larvae were studied by film-coating technique. [Results] The incorporation of R. glutinosa root or leaf dry powder into the larvae’s diet notably affected their growth and development. An increase of the root or leaf dry power content in the diet led to higher larval mortality, extended developmental periods, and decreased body weight. The most significant inhibitory effect occurred when the mass ratio of root or leaf dry powder to feed was 1 ∶3. The ethanol extracts of R. glutinosa root or leaf exhibited a certain inhibitory effect on the younger larvae, while with diminished efficacy on older larvae. As the concentration of R. glutinosa extract increased, the mortality of younger larvae increased at the same testing period. Optimal toxicological effects on 1st to 6th instar larvae were observed when the root or leaf dry power of R. glutinosa to solvent ratio was 1 ∶10.[Conclusion] R. glutinosa shows a definitive inhibitory effect on S. litura. In a certain range, the greater dosage of R. glutinosa root or leaf dry powder has the stronger inhibitory effect. This finding lays a theoretical groundwork for the strategic utilization of agricultural weed resources in developing plant-derived insecticides.

【基金】 国家现代农业产业技术体系棉花产业技术体系(CARS-15-22)
  • 【分类号】S435.62
  • 【下载频次】10
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