【摘要】 [目的]探索水松根际微生物群落结构和功能及随季节变化的规律。[方法]采用PCR-DGGE和Biolog-ECO微平板法测定不同季节水松根际土壤理化性质、微生物群落结构和功能，通过主成分分析和冗余分析等方法解析根际微生态各环境因子的内在规律。[结果]水松根际微生物群落中优势细菌为α-变形菌（Alphaproteobacteria）、酸杆菌（Acidobacteria）和δ-变形菌（Deltaproteobacteria），优势真菌为粪壳菌（Sordariomycetes）。夏季水松根际微生物量和微生物多样性最高。根际微生物的碳代谢能力在夏季最强，冬季最弱，大致表现为夏季>秋季>春季>冬季，速效氮可能是微生物群落碳代谢功能的主要驱动因子。[结论]水松根际微生物群落多样性和碳代谢能力在夏季最优，在冬季最差。季节因素显著影响着根际微生物群落结构和功能，开展相关研究时应充分考虑季节因素造成的波动。更多还原

【Abstract】 [Objective] To explore the structure and function of rhizosphere microbial community of Glyptostrobus pensilis and its seasonal variation. [Methods] PCR-DGGE and Biolog-ECO microplate methods were used to determine the soil physicochemical properties, structure and function of rhizosphere microbial community of G. pensilis in different seasons, and the intrinsic patterns of rhizosphere microecological factors were analyzed by principal component analysis and redundancy analysis. [Result] The dominant bacterial in the rhizosphere microbial community of G. pensilis were Alphaproteobacteria, Acidobacteria and Deltaproteobacteria, and the dominant fungi was Sordariomycetes. Rhizosphere microbial biomass content and microbial diversity in the soil of G. pensilis are the highest in summer. Carbon metabolism of rhizosphere microorganisms in the summer was the largest, followed by autumn, spring, and winter. Available nitrogen may be the main factor driving changes in the functional diversity of carbon metabolism in rhizosphere microbial communities. [Conclusion] The diversity and carbon metabolism ability of rhizosphere microbial community of G. pensilis are the best in summer and the worst in winter. Seasonal factors significantly affect the structure and function of rhizosphere microbial community, and the fluctuation caused by seasonal factors should be fully considered in the future related research.更多还原