【摘要】 为了鉴定奶牛小肠上皮细胞系(BIECs-21)的生物学遗传稳定性，体外贴壁培养BIECs-21细胞，制备染色体标本和G-带标本，分析BIECs-21细胞的染色体核型和G-带。结果表明：BIECs-21细胞染色体数为2n=60,包括常染色体29对和性染色体(X和Y染色体)1对。其中，29对常染色体均为端部着丝点染色体；性染色体中，X染色体是较大的中着丝粒染色体，Y染色体是较小的中着丝粒染色体。除X、Y性染色体外，BIECs-21细胞常染色体的G-带带纹的数量和位置无显著差异。BIECs-21细胞与荷斯坦牛的正常染色体核型相比，染色体数目及形态结构均没有明显变化，证明该细胞系遗传特性稳定，可以用于牛肠道相关疾病分子机制的研究。更多还原

【Abstract】 In order to identify the genetic stability in biology of bovine small intestinal epithelial cell line(BIECs-21), BIECs-21 cells were cultured in vitro by using adherent culture, and chromosome and G-band samples were prepared to analyze the chromosome karyotype and G-band of BIECs-21 cells. The results show that the number of chromosomes in BIECs-21 cells is 2n = 60, including 29 pairs of autosomes and 1 pair of sex chromosomes(X and Y chromosomes). Among them, 29 pairs of autosomes are all terminal centromere chromosomes, with X chromosome being the larger centromere chromosome and Y chromosome being the smaller centromere chromosome in the sex chromosome. There is no significant difference in the number and position of G-bands pattern on the autosomes of BIECs-21 cells, except for X and Y sex chromosome. Compared with the normal chromosome karyotype of Holstein cattle, there is no significant change in the number and morphological structure of chromosomes in BIECs-21 cells, indicating that the genetic characteristics of the cell line are stable, and it can be used for the reaearch on the molecular mechanisms of bovine intestinal related diseases.更多还原