【摘要】 为检测荞麦类食品及原料中的荞麦源性成分，根据荞麦品种DW-1核酸序列信息(KY945277.1)设计了荞麦品种特异性引物；以苦荞和甜荞品种基因组为模板，建立了荞麦源性成分的实时荧光PCR(qPCR)的检测方法，并对购自市场的多个粮食产品进行检测，检测结果与商品标识相符方法特异性强、灵敏度高，为食品中荞麦源性成分的检测提供了新思路。更多还原

【Abstract】 In order to detect buckwheat derived components in buckwheat food and raw materials, buckwheat variety-specific primers were designed according to the DW-1 nucleic acid sequence information of buckwheat variety(KY945277.1). A realtime fluorescence PCR(qPCR) method for the detection ofsweet buckwheat derived components was established using the genome of tartary buckwheat and buckwheat varieties as templates. Multiple food products purchased from the market were tested, and the detection results were consistent with the product labeling. The method has strong specificity and sensitivity, providing a new idea for the detection of buckwheat derived components in food.更多还原