【摘要】 通过分散液液微萃取-高效液相(DLLME-HPLC)建立一种同时测定食用植物油中对羟基肉桂酸和橙皮素的分析方法。以正己烷为分散剂，以含体积分数5%氨水(500μL)和体积分数10%乙腈的水溶液为萃取剂萃取样品，以体积分数0.3%三氟乙酸溶液-乙腈为流动相，在柱温25℃条件下进行梯度洗脱，萃取液放置过夜后再进行测定。结果表明：样品峰面积的相对标准偏差均小于3%,对羟基肉桂酸和橙皮素在各自质量浓度范围内的线性相关系数均大于0.99,检测限分别为8.7、6.2 ng/g,定量限分别为26.1、18.5 ng/g,回收率分别为93.1%～103.7%、100.5%～111.1%。更多还原

【Abstract】 A method for simultaneous determination of the content of p-Hydroxy-cinnamic acid and hesperidin in edible vegetable oil was established by dispersive liquid-liquid microextraction-high-performance liquid chromatography(DLLME-HPLC). The samples were extracted using n-hexane as dispersant and aqueous solution containing volume fraction 5 % ammonia solution(500 μL) and volume fraction 10 % acetonitrile as the extractant. Using volume fraction 0.3 %trifluoroacetic acid solution-acetonitrile as the mobile phase, gradient elution was performed at column temperature 25 ℃, and the extraction solution was left overnight before measurement.The results showed that the relative standard deviation of the peak area of the samples was less than 3 %, and the linear correlation coefficients of p-Hydroxy-cinnamic acid and hesperidin within their respective mass concentration ranges were greater than 0.99, and their detection limits were 8.7 and 6.2 ng/g, quantitative limits were 26.1 and 18.5 ng/g, and recovery rates were 93.1 %-103.7 % and 100.5 %-111.1 %, respectively.更多还原