【摘要】 试验旨在探讨复方中草药防治虾夷扇贝脓疱病的可行性，从患脓疱病虾夷扇贝的病灶部位分离纯化病原菌，通过体外试验检测复方中草药的抑菌效果，并通过人工回感试验检测复方中草药对该病原菌的防治效果。结果显示，经生理生化及分子生物学鉴定，分离病原菌为海藻希瓦氏菌（Shewanella algae）。该菌株对头孢曲松、氨曲南、氧氟沙星等10种药物高度敏感，对头孢西汀和头孢噻吩不敏感。复方中草药对该菌株的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）分别为0.625 g/L和1.25 g/L。饲料中添加复方中草药能够显著降低虾夷扇贝体内细菌量（P<0.05），显著提高虾夷扇贝抗氧化酶活性（P<0.05），降低虾夷扇贝死亡率。研究表明，可采用在饲料中添加复方中草药的方式防治海藻希瓦氏菌诱发的虾夷扇贝脓疱病，具有良好的应用前景。更多还原

【Abstract】 The purpose of this study was to investigate the feasibility of compound Chinese herbal medicine for preventing and treating impetigo of Patinopecten yessoensis. The pathogenic bacteria were isolated and purified from the lesion site of Patinopecten yessoensis. The antibacterial effect of the compound Chinese herbal medicine was detected by in vitro test,and the control effect of the compound Chinese herbal medicine on the pathogenic bacteria was detected by artificial response test. The results showed that the pathogen was identified as Shewanella algae by physiological, biochemical and molecular biology. The strain was highly sensitive to 10 drugs such as ceftriaxone, amtronam and ofloxacin, but was not sensitive to sporacetine and cefalothiophene. The MIC and MBC of compound Chinese herbal medicine were 0.625 g/L and 1.25 g/L, respectively. The addition of compound Chinese herbal medicine in the diet could significantly reduce the bacteria content in the body of Patinopecten yessoensis(P<0.05), significantly increase the antioxidant enzyme activity of Patinopecten yessoensis(P<0.05), and reduce the mortality of Patinopecten yessoensis. The study showed that the method of adding compound Chinese herbal medicine to the feed can prevent and control the impetigo of Patinopecten yessoensis induced by Shewanella algae, which is a good application prospect.更多还原