【摘要】 【目的】为开发新的森林病害的微生物生防制剂，探明和优化真菌生防菌株AH-1的固体发酵条件。【方法】使用单因素测试及响应面法优化发酵条件。【结果】结果表明，影响固体发酵的3个主要因素是接种量、麦麸含量和温度；优化后的固体发酵培养基组成为麦麸∶玉米粉约3∶2。优化后的固体发酵培养条件为：初始pH值7、含水量60%，粘帚菌种子液接种量约18%（孢子浓度为1×10⁶个/mL），温度30℃，发酵天数10 d。此条件下的产孢量为1.672×10¹⁰个孢子/g，较优化前的单因素试验产孢量提高约2～3倍。【结论】用响应曲面法优化的发酵条件，能明显提高固体发酵的生防菌株AH-1的产孢量，为其规模化生产和菌剂制备奠定了基础。更多还原

【Abstract】 【Objective】In order to develop new biocontrol agent, the conditions of solid-state fermentation of biocontrol strain AH-1 were explored and optimized.【Methods】 Singal factor assessment and response surface methodology were performed.【Result】The results showed that the three main factors affecting solid-state fermentation were inoculation amount, wheat bran content and temperature. The optimized solid-state fermentation medium composition was wheat bran : corn flour of 3 : 2. The optimized solid-state fermentation culture conditions were as follows: initial pH-value of 7, water content of 60%, spore suspension inoculation amount of about 18%（with concentration of 1×10⁶ conidia/mL）, temperature of 30 °C, and fermentation period of 10 d.Under these conditions, the conidial yield was 1.672×10¹⁰ conidia/g, which was about 2～3 times higher than that of the pre-optimization factor test.【Conclusion】 The fermentation conditions optimized by the response surface method could obviously increase the sporulation of biocontrol strain AH-1 in solid-state fermentation, which laid a foundation for its large-scale production and preparation of microbial agents.更多还原