【摘要】 目的 探讨结直肠癌组织中PKD1和PKD2的表达及临床意义。方法 收集黑龙江省林业第二医院2019年1月至2020年12月收治的90例结直肠癌患者的癌组织及对应癌旁组织。免疫组化染色法检测PKD1和PKD2的表达，分析PKD1和PKD2表达与结直肠癌临床病理特征的关系。Kaplan-Meire法绘制生存曲线，生存差异行Log-rank检验。Cox风险比例回归模型分析影响结直肠癌预后的因素。结果 结直肠癌组织中PKD1阳性率为72.2%(65/90),高于癌旁组织的31.1%(28/90),差异有统计学意义(P<0.05); PKD2阳性率为66.7%(60/90),高于癌旁组织的33.3%(30/90),差异有统计学意义(P<0.05)。PKD1、PKD2表达与分化程度、TNM分期、浸润深度和淋巴结转移有关(P<0.05)。Kaplan-Meier分析显示，PKD1阴性患者中位总生存(OS)为34.0个月(31.5～36.0个月),显著高于PKD1阳性患者的20.0个月(17.3～26.7个月),差异有统计学意义(P<0.05)。PKD2阴性患者中位OS为33.0个月(25.6～36.2个月),显著高于PKD2阳性患者的21.0个月(19.1～27.2个月),差异有统计学意义(P<0.05)。多因素Cox风险比例回归模型分析显示，分化程度、TNM分期、淋巴结转移、PKD1表达和PDK2表达是影响结直肠癌患者OS的独立因素(P<0.05)。结论 结直肠癌组织中PKD1和PKD2高表达，PKD1和PKD2阳性表达可作为评估结直肠癌患者预后不良的独立因素。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of PKD1 and PKD2 in colorectal cancer.Methods Cancer tissues and corresponding para-cancer tissues of 90 colorectal cancer patients admitted to the Second Forestry Hospital of Heilongjiang Province from January 2019 to December 2020 were collected. The expression of PKD1 and PKD2 was detected by immunohistochemical staining, and the relationship between PKD1 and PKD2 expression and clinicopathological features of colorectal cancer was analyzed. Survival curve was plotted by Kaplan-Meire method. Cox risk proportional regression model was used to analyze the prognostic factors of colorectal cancer.Results The positive rate of PKD1 in colorectal cancer tissues was 72.2%(65/90), higher than 31.1%(28/90) in adjacent tissues, the difference was statistically significant(P<0.05). The positive rate of PKD2 was 66.7%(60/90), higher than that of para-cancer tissues [33.3%(30/90)], the difference was statistically significant(P<0.05). The expressions of PKD1 and PKD2 were correlated with differentiation degree, TNM stage, invasion depth and lymph node metastasis(P<0.05). Kaplan-Meier analysis showed that the median overall survival(OS) of PKD1-negative patients was 34.0 months(31.5-36.0 months), which was significantly higher than that of positive patients, and the difference was statistically significant(P<0.05). The median OS of PKD2-negative patients was 33.0 months(25.6-36.2 months), which was significantly higher than that of positive patients, and the difference was statistically significant(P<0.05). Multivariate Cox proportional regression analysis showed that differentiation degree, TNM stage, lymph node metastasis, PKD1 expression and PDK2 expression were independent factors affecting OS in colorectal cancer patients(P<0.05).Conclusion PKD1 and PKD2 are highly expressed in colorectal cancer tissues, and positive expression of PKD1 and PKD2 can be used as an independent factor to evaluate the poor prognosis of patients with colorectal cancer.更多还原