【摘要】 目的 分析食管鳞癌(ESCC)中乳腺癌易感基因1相关环指结构域蛋白1(BARD1)的表达及与淋巴结转移的关系。方法 纳入2018年1月至2021年12月本院收治且经组织病理学检查确诊的114例ESCC患者，免疫组化EnVision两步法检测癌组织与癌旁组织中BARD1的表达情况并比较BARD1阳性率，分析BARD1表达与ESCC临床病理特征的关系，建立Logistic回归模型分析BARD1表达对ESCC淋巴结转移的影响，绘制受试者工作特征(ROC)曲线分析BARD1表达对ESCC淋巴结转移的预测价值。结果 114例ESCC组织中BARD1阳性78例、阴性36例，对应癌旁组织中BARD1阳性23例、阴性91例，ESCC组织的BARD1阳性率为68.42%,高于癌旁组织的20.18%(χ~2=53.769,P<0.001)。BARD1表达与ESCC患者的T分期、分化程度和淋巴结转移有关(P<0.05),其中淋巴结转移组织的BARD1阳性率为80.36%(45/56),高于未转移组织的56.90%(33/58)。经Phi系数分析发现，BARD1表达与ESCC淋巴结转移呈正相关(r=0.276,P=0.003)。BARD1阳性是ESCC患者淋巴结转移的独立危险因素(OR=3.099,95%CI:1.339～7.175,P=0.008),且BARD1表达对ESCC患者淋巴结转移具有一定预测价值(AUC=0.636,P=0.020)。结论 BARD1在ESCC中高表达，其与ESCC淋巴结转移密切相关，BARD1阳性会增加淋巴结转移风险，在评估ESCC淋巴结转移上有一定价值。更多还原

【Abstract】 Objective To analyze the expression of breast cancer susceptibility gene 1-associated ring structural domain protein 1(BARD1) in esophageal squamous cell carcinoma(ESCC) tissues and its correlation with lymph node metastasis. Methods From January 2018 to December 2021, 114 patients with ESCC diagnosed by histopathological examination in our hospital were included. Immunohistochemical EnVision two-step method was used to detect the BARD1 expression in cancer tissues and adjacent tissues, and then positive rates of BARD1 were compared. Relationship between the BARD1 expression and the clinicopathological features of ESCC was evaluated. Logistic regression model was established to analyze the influence of BARD1 expression on lymph node metastasis of ESCC, and receiver operating characteristic(ROC) curve was used to determine the predictive value of BARD1 expression on lymph node metastasis of ESCC. Results Among 114 ESCC tissues, 78 were positive and 36 were negative for BARD1, corresponding to 23 positive and 91 negative for BARD1 in adjacent cancerous tissues. The positive rate of BARD1 in ESCC tissues was 68.42%, higher than the 20.18% in adjacent tissues(χ~2=53.769,P<0.001). BARD1 expression was related to T stage, differentiation degree, and lymph node metastasis in ESCC patients(P<0.05), with a positive rate of BARD1 in lymph node metastasis tissues of 80.36%(45/56), higher than 56.90%(33/58) in non-metastatic tissues. Through Phi coefficient analysis, it was found that BARD1 expression was positively correlated with lymph node metastasis in ESCC(r=0.276, P=0.003). BARD1 positivity was an independent risk factor for lymph node metastasis in ESCC patients(OR=3.099, 95% CI: 1.339-7.175, P=0.008), and BARD1 expression had certain predictive value for lymph node metastasis in ESCC patients(AUC=0.636, P=0.020). Conclusion BARD1 is highly expressed in ESCC and is closely related to lymph node metastasis in ESCC. Positive BARD1 increases the risk of lymph node metastasis and has certain value in evaluating lymph node metastasis in ESCC.更多还原