【摘要】 目的 探讨儿童AL的治疗方法。方法 采用Ficoll-Hypaque法分离AL患儿MNC,将其分为4组。对照组：以含10%FCS的RPMI1640培养液培养；实验1组：单用MDP;实验2组：rhGM-CSF+rhIL-4+rhTNF-α;实验3组：rhGM-CSF+rhIL-4+rhTNF-α+MDP。观察每日细胞生长情况及细胞形态，培养8d后计数各组细胞数量并应用流式细胞仪检测各组细胞免疫表型。结果 对照组培养3 d,细胞大量死亡，数量明显减少；而实验各组在培养第2-8天均出现细胞形态的变化；实验各组细胞数及HLA-DR、CD1a、CD83细胞比例均高于对照组，以实验3组最高(P<0.05)。结论 MDP对AL患儿骨髓DC有扩增作用并促其成熟，与细胞因子联合作用更强。更多还原

【Abstract】 Objective To explore the treatment methods for children with AL.Methods The Ficoll Hypaque method was used to isolate MNCs from AL patients, who were divided into four groups. Control group: cultured in RPMI1640 medium containing 10% FCS; Experiment 1 group: using MDP alone; Experiment 2 group: rhGM-CSF+rhIL-4+rhTNF-α; Experiment 3 group: rhGM-CSF+rhIL-4+rhTNF-α+MDP.Observe daily cell growth and morphology, count the number of cells in each group after 8 days of culture, and use flow cytometry to detect the immune phenotype of each group of cells.Results After 3 days of cultivation in the control group, a large number of cells died and the quantity significantly decreased; And all experimental groups showed changes in cell morphology from day 2 to day 8 of cultivation; The number of cells and the proportion of HLA DR, CD1a, and CD83 cells in each experimental group were higher than those in the control group, with the highest in experimental group 3(P<0.05). Conclusion MDP has an amplifying effect on bone marrow DCs in children with AL and promotes their maturation, with a stronger synergistic effect with cytokines.更多还原