【摘要】 采用超声波低温提取结合大孔树脂吸附分离技术从马蓝叶中提取富集吲哚苷，在单因素试验基础上通过响应面法优化超声波提取工艺，从6种大孔树脂中筛选最适类型并确定大孔树脂纯化吲哚苷的吸附/解吸最佳工艺条件，制备高纯度吲哚苷提取物；采用96孔板微量法测定了高纯度吲哚苷对金黄色葡萄球菌和大肠杆菌的抑菌活性。研究结果表明：超声波提取的最佳工艺条件为5 g干燥马蓝叶粉末、超声波功率458 W、提取时间29 min、温度32℃、料液比1∶10(g∶mL)、提取2次，在此条件下马蓝叶吲哚苷提取率为(93.15±0.26)%。X-5型大孔树脂对吲哚苷吸附/解吸效果最好，大孔树脂纯化最优条件为：大孔树脂100 g、提取液上样量500 mL、洗脱液乙醇体积分数为40%、洗脱液体积400 mL,该条件下吲哚苷回收率为(82.97±0.30)%,纯度由(18.32±0.18)%提升至(86.43±0.31)%;吲哚苷粗提物对金黄色葡萄球菌和大肠杆菌的最小抑菌质量浓度(MIC)值均为350 mg/L,经纯化后的高纯度吲哚苷提取物对金黄色葡萄球菌和大肠杆菌的MIC值分别为21.88和43.75 mg/L,显示出比阳性对照硫酸庆大霉素(MIC值分别为43.75和87.50 mg/L)更好的抑菌效果。更多还原

【Abstract】 In this study, indican was extracted and enriched from the leaves of Baphicacanthus cusia(Ness) Kuntze using ultrasonic low-temperature extraction and adsorption-separation technology with macroporous resin. The ultrasonic extraction process was optimized by response surface methodology based on the single-factor experiment. The optimal type was selected from six kinds of macroporous resins and the optimal adsorption-desorption process conditions of macroporous resin purification of indican were determined. Meanwhile, the high-purity indican extract was prepared. The minimum inhibitory concentration(MIC) of high-purity indican for Staphylococcus aureus and Escherichia coli was determined using 96 well plate micro method. The results showed that the optimal conditions for ultrasonic extraction process were as follows: the dried B.cusia leaf powder was 5 g, the ultrasound power was 458 W, the extraction time was 29 min, the temperature was 32 ℃, the solid-liquid ratio was 1 ∶10(g ∶mL), and extracted 2 times. Under these conditions, the extraction rate of indican from B. cusia leaves was(93.15±0.26)%. The X-5 type of microporous resin had the best adsorption-desorption performance of the indican. The optimal process conditions of purification with macroporous resin were as follows: macroporous resin was 100 g, the sample volume was 500 mL, the ethanol volume fraction in the eluent was 40%, and the elution volume was 400 mL. Under these conditions, the recovery rate of indician was(82.97±0.30)%, and the purity increased from(18.32±0.18)% to(86.43±0.31)%. The MIC values of indican crude extract against S. aureus and E. coli both were 350 mg/L. The purified indician exhibited excellent antibacterial activity. The MIC values of high-purity indican extract against S. aureus and E. coli were 21.88 and 43.75 mg/L, respectively, which presented better antibacterial effect than the positive control gentamycin sulfate(MIC values were 43.75 and 87.50 mg/L).更多还原