【摘要】 通过纤维素酶辅助法水解油茶饼粕提取糖萜素，以糖萜素得率作为考察目标，通过正交试验，探究纤维素酶用量、提取时间、料液比、提取温度、pH值对糖萜素得率的影响。正交试验结果表明：在料液比为1:15(g:mL),纤维素酶用量为4%、酶解pH值为5.0、提取温度为50℃、提取时间为120 min的最优条件下，糖萜素的得率为18.39%,经红外光谱(FT-IR)和高效液相色谱(HPLC)检测发现提取物符合糖萜素的基本结构特征。参照GB/T 25247—2010国家标准方法，对糖萜素进行了鉴别及成分测定，总糖和总皂苷质量分数均大于30%,蛋白质质量分数大于7%。糖萜素具有较好的DPPH自由基(DPPH·)清除能力，质量浓度为1 g/L时，对DPPH·的清除率达到90.28%;且在质量浓度小于5 mg/L时没有溶血活性，有作为饲料添加剂使用的潜在可能。更多还原

【Abstract】 The extraction of saccharicterpenin from Camellia oleifera cake meal by cellulase hydrolysis method was studied, with the saccharicterpenin yield as the investigation target. An orthogonal experiment was conducted to explore the effects of cellulase dosage, extraction time, liquid-to-solid ratio, extraction temperature, and pH value on the saccharicterpenin yield. The results showed that under the optimal experimental conditions of a solid-to-liquid of 1 :15(g :mL), cellulase dosage of 4%, enzymatic hydrolysis pH value of 5.0, extraction temperature of 50 ℃, and extraction time of 120 min, the saccharicterpenin yield was 18.39%. The extract was found to conform to the basic structural characteristics of saccharicterpenin through infrared spectroscopy and high-performance liquid chromatography. Identification and component determination of saccharicterpenin were performed according to the national standard method of GB/T 25247—2010. The mass fractions of total sugars and total saccharicterpenin were more than 30%, and the mass fraction of proteins exceeded 7%. Saccharicterpenin exhibited good DPPH radical scavenging ability, with a clearance rate of 90.28% at a mass concentration of 1 g/L. And there was no hemolytic activity at mass concentration less than 5 mg/L, which had good prospects for application as a feed additive.更多还原