【摘要】 目的 通过旷场实验、免疫组化染色，研究GsMTx4在牙齿移动过程中对大鼠痛觉的抑制作用。方法 选取58只5～6周雄性SD大鼠，并构建右上颌牙齿移动模型。随机将大鼠分为A组(对照组)、B组(GsMTx4组)两组，另按加力时间4 h和1、3、5、7 d分为5个亚组，以A组大鼠左上颌作为C组(空白对照组)。A、B两组大鼠分别局部注射生理盐水、GsMTx4。通过旷场实验评价大鼠的疼痛程度，免疫组化染色观察牙周组织中神经生长因子(nerve growth factor, NGF)、降钙素基因相关肽(calcitonin gene-related peptide, CGRP)的表达情况。结果 4 h、1 d、3 d时，B组大鼠在旷场中5 min内的总路程较A组更高(P<0.05)。在4 h、1 d时，B组大鼠中心区域停留时间百分比较A组高(P<0.05)。A、B两组中NGF、CGRP的表达在1、3、5、7 d时高于C组(P<0.05)。B组NGF、CGRP表达在4 h、1 d、3 d的均低于A组(P<0.05)。结论 GsMTx4在牙齿移动过程中能抑制NGF、CGRP的表达，并缓解牙齿移动时产生的疼痛。更多还原

【Abstract】 Objective To investigate the inhibitory effect of GsMTx4 on pain sensation in rats during tooth movement, open field experiments and immunohistochemical staining by conducting. Methods Fifty-eight male SD rats aged 5-6 weeks were selected and a right maxillary tooth movement model was constructed. The rats were randomly divided into two groups: group A(control group) and group B(GsMTx4 group). They were also divided into 5 subgroups according to 4 h, 1 d, 3 d, 5 d, and 7 d. The left maxillary of the rats in group A was used as group C(blank control group). The open-field test was used to evaluate the pain level of rats. Immunohistochemical staining was used to observe the expression of nerve growth factor(NGF) and calcitonin gene-related peptide(CGRP) in periodontal tissues. Results At 4 h, 1 d, and 3 d, the total distance traveled by rats in group B within 5 minutes in the open-field was higher than that of group A(P<0.05). At 4 h and 1 d, the percentage of time in the center of rats in group B was higher than that in group A(P<0.05). The expressions of NGF and CGRP in groups A and B were higher than those in group C on 1 d, 3 d, 5 d, and 7 d(P<0.05). The expression of NGF and CGRP in group B were lower than those in group A at 4 h, 1 d, and 3 d(P<0.05). Conclusion GsMTx4 can inhibit the expression of NGF and CGRP during tooth movement and relieve the pain caused by tooth movement.更多还原