【摘要】 目的 探讨银纳米粒子涂层根管镍钛器械(silver nanoparticals-coated root canal nickel titanium instruments, AgNPs-NiTi)的抗菌性能及生物相容性。方法 利用脉冲电化学沉积技术制备AgNPs-NiTi。场发射扫描电镜观察AgNPs-NiTi的形貌，X射线衍射仪和能谱仪分析涂层的元素组成及含量，万能材料实验机检测AgNPs-NiTi的机械性能。将AgNPs-NiTi与粪肠球菌(E.faecalis)悬液共培养，细菌菌落计数法检测AgNPs-NiTi对E.faecalis的抗菌效果。构建离体牙根管内E.faecalis生物膜模型，将AgNPs-NiTi放入根管内模拟器械分离，用场发射电镜、活死菌染色法观察AgNPs-NiTi对E.faecalis生物膜的抗菌效果。AgNPs-NiTi与Raw264.7细胞共培养，用CCK-8法检测其细胞毒性。结果 脉冲电化学沉积法在NiTi器械上构建银纳米粒子涂层，载银后NiTi器械的机械性能无明显变化。AgNPs-NiTi能够明显抑制E.faecalis的增殖，并显著破坏根管内E.faecalis生物膜。AgNPs-NiTi对Raw264.7的增殖无明显影响，无明显细胞毒性。结论 AgNPs-NiTi的机械性能与镍钛器械相仿，可抑制E.faecalis及其生物膜的活性，具有良好的生物相容性。更多还原

【Abstract】 Objective To investigate the antibacterial performance and biocompatibility of silver nanoparticles-coated root canal nickel titanium instruments(AgNPs-NiTi). Methods AgNPs-NiTi was prepared using pulse electrochemical deposition. The morphology of AgNPs-NiTi was observed using field emission scanning electron microscopy(FE-SEM), and the elemental composition and content were analyzed using X-ray diffraction(XRD)and energy dispersive spectroscopy(EDS). The mechanical properties of AgNPs-NiTi were tested. After Co-culturing AgNPs-NiTi with E. faecalis, the antibacterial effect was detected by colony-forming units method. By constructing an in vitro model of E. faecalis biofilm in the root canal of teeth, the antibacterial effect of AgNPs-NiTi was observed using FE-SEM and live/dead bacterial staining. In addition, AgNPs-NiTi was co-cultured with Raw 264.7 cells, and its cytotoxicity was detected by CCK-8. Results The pulse electrochemical deposition was used to construct a silver nanoparticle(AgNPs)coating on NiTi instruments with no significant change in the mechanical properties. AgNPs-NiTi significantly inhibited the proliferation of E. faecalis and damaged E. faecalis biofilm in the root canal. AgNPs-NiTi had no significant influence on the proliferation of Raw264.7 cells and had no cytotoxicity. Conclusion The mechanical properties of AgNPs-NiTi are similar to those of nickel titanium instruments. AgNPs-NiTi inhibits E. faecalis proliferation with good biocompatibility.更多还原