【摘要】 目的:探索甲基丙烯酰氧基癸基磷酸二氢酯（MDP）-钙盐对牙髓干细胞（DPSCs）成牙本质分化的影响。方法:使用不同比例的10-MDP与CaCl₂合成三种MDP-钙盐；提取人DPSCs,通过CCK-8实验研究不同浓度MDP-钙盐对细胞增殖的影响，碱性磷酸酶（ALP）活力测试选取最适浓度，茜素红染色和实时荧光定量PCR研究MDP-钙盐对DPSCs成牙本质分化的影响。结果:一定浓度范围MDP-钙盐对DPSCs增殖无抑制作用，MDP-钙盐促进DPSCs的ALP活力的最佳浓度为0.2 mg/mL（P<0.05）,0.2 mg/mL MDP-2钙盐促进DPSCs钙结节形成（P<0.05）,0.2 mg/mL MDP-1钙盐和MDP-2钙盐对DPSCs中牙本质基质蛋白-1（DMP-1）的分泌有促进作用（P<0.05）,0.2 mg/mL MDP-0.5钙盐和MDP-1钙盐对DPSCs中牙本质涎磷蛋白（DSPP）的分泌有促进作用（P<0.05）,0.2 mg/mL MDP-0.5钙盐和MDP-2钙盐对DPSCs中骨钙素（OCN）的分泌有促进作用（P<0.05）,0.2 mg/mL MDP-0.5钙盐、MDP-1钙盐和MDP-2钙盐对DPSCs中Runt相关转录因子2（RUNX2）的分泌有促进作用（P<0.05）。结论:MDP-钙盐在一定浓度范围内对DPSCs增殖无抑制作用，0.2 mg/mL MDP-钙盐对DPSCs成牙本质分化有促进作用。更多还原

【Abstract】 Objective: To investigate the effects of methacryloyloxydecyl dihydrogen phosphate（MDP）-calcium salts on the proliferation and odontogenic differentiation of dental pulp stem cells（DPSCs）. Methods: Three types of MDP-calcium salts were synthesized using different ratios of 10-MDP and CaCl₂, and Human DPSCs were harvested and cultured in medium. The effect of different concentrations of MDP-calcium salts on cell proliferation was determined by CCK-8 assay. Alkaline phosphatase（ALP） activity was measured to screen the best concentration for subsequent experiments. Additionally, alizarin red staining, and real-time quantitative polymerase chain reaction（qRT-PCR） were employed to study the influence of a specific concentration of MDP-calcium salts on the odontogenic differentiation of DPSCs. Results: A certain concentration range of MDP-calcium salts had no inhibitory effect on the proliferation of DPSCs. Compared with the control group, 0.2 mg/mL was the optimal concentration of MDP-calcium salts in inducing ALP activity of DPSCs（P<0.05）. Alizarin red staining results showed that 0.2 mg/mL MDP-2 calcium salts promoted the formation of calcium nodules in DPSC 0.2 mg/mL MDP-1 calcium salts and 0.2 mg/mL MDP-2 calcium salts promoted the secretion of odontogenic differentiation marker dental matrix protein-1（DMP-1）（P<0.05）. Additionally, 0.2 mg/mL MDP-0.5 calcium salts and 0.2 mg/mL MDP-1 calcium saltsenhance the secretion of dentin sialophosphoprotein（DSPP）（P<0.05）. Furthermore, 0.2 mg/mL MDP-0.5 calcium salts and 0.2 mg/mL MDP-2 calcium salts also enhanced the secretion of osteocalcin（OCN）（P<0.05）. Lastly, 0.2 mg/mL MDP-0.5 calcium salts, 0.2 mg/mL MDP-1calcium salts and 0.2 mg/mL MDP-2 calcium salts stimulated the secretion of runt-related transcription factor 2（RUNX2）（P<0.05）. Conclusions: MDP-calcium salts can not inhibit the proliferation of DPSCs within a certain concentration range. 0.2 mg/mL MDP-calcium salts can promote the odontogenic differentiation of DPSCs.更多还原