【摘要】 本文通过扫描电镜（SEM）、氨基酸分析、傅立叶红外光谱（FTIR）、X射线晶体衍射（XRD）和热重分析（TG）等技术对二化螟盘绒茧蜂Cotesia chilonis茧丝纤维的微观形态、氨基酸类别与组成、二级结构和热稳定性等作了分析。结果表明，二化螟盘绒茧蜂的茧丝纤维表面较为粗糙，直径为1.89±0.04μm。茧丝纤维主要由碳（60.67%）、氮（21.17%）和氧（17.22%）等元素组成，其丝蛋白主要成分包括天冬氨酸/天冬酰胺、丝氨酸和丙氨酸等；蛋白二级结构主要为β-折叠；其峰值降解温度为332.27℃±2.58℃。本文研究揭示了二化螟盘绒茧蜂茧丝纤维的结构特征与理化性能，为拓展该蜂的应用领域、开发性能优越的非蚕丝蛋白以及设计改造新型丝蛋白纤维提供基础。更多还原

【Abstract】 In this paper, the morphological structure, amino acid composition, secondary structure and thermal stability of silk fibers from the cocoons of the parasitoid wasp, Cotesia chilonis(Matsumura)(Hymenoptera: Braconidae) were analyzed by the scanning electron microscopy(SEM), amino acid analysis, Fourier transform infrared spectroscopy(FTIR), X-ray crystallography(XRD) and thermogravimetric analysis(TG). The results showed that the surface of the silk fiber from C. chilonis cocoons was relatively rough, and its diameter was 1.89 ± 0.04 μm. It was mainly composed of elements such as carbon(60.67%), nitrogen(21.17%), and oxygen(17.22%). The protein components of C. chilonis cocoons mainly included aspartic acid/asparagine, serine and alanine. The secondary structure of silk proteins from C. chilonis cocoons was mainly β-sheet, and its peak degradation temperature was 332.27 ±2.58℃. This study reveals the ultrastructural characteristics and physicochemical properties of silk fibers from C. chilonis cocoons, which provides a basis for expanding the application field of C. chilonis,developing non-worm silk proteins with superior performance, and designing and transforming new silk protein fibers.更多还原