【摘要】 目的 ：研究CXC趋化因子配体8(CXC chemokine ligand 8,CXCL8)在胃肠道间质瘤（gastrointestinal stromal tumors,GIST）中的表达以及临床意义。方法：通过生物信息学分析耐药和敏感GIST组织转录组的差异基因，绘制差异基因的韦恩图和火山图，筛查差异最明显的基因。收集4例伊马替尼耐药GIST组织标本和4例敏感GIST组织标本，采用免疫组织化学法检测CXCL8蛋白的表达。将88例GIST患者分为CXCL8高表达组49例和低表达组39例，比较两组患者临床病理参数。绘制Kaplan-Meier生存曲线，比较CXCL8高表达与低表达患者总生存期（overall survival,OS）及无病生存期（disease-free survival,DFS）。采用单因素和多因素Cox回归模型分析影响患者生存期的因素。结果：生物信息学分析显示，耐药GIST组织中CXCL8基因表达高于敏感组织。免疫组织化学结果显示，耐药GIST组织中CXCL8蛋白表达显著高于敏感组织（P<0.05）。CXCL8蛋白表达与肿瘤最大直径、核分裂相计数以及危险度分级有关（P<0.05）。Kaplan-Meier生存曲线显示，CXCL8高表达患者DFS低于低表达患者（P<0.05）。Cox回归模型分析显示，CXCL8高表达是影响GIST患者DFS的独立危险因素。结论：CXCL8促进GIST耐药，与GIST患者的预后相关，可能成为胃肠道间质瘤特异性治疗的新靶点。更多还原

【Abstract】 Objective:To investigate the expression and clinical significance of CXC chemokine ligand 8(CXCL8) in gastrointestinal stromal tumors(GIST). Methods:The differential genes of drug-resistant and sensitive tissue transcriptomes were analyzed through bioinformatics, and the Venn diagram and volcano diagram of differential genes were drawn to find out the genes with the most obvious differences. Immunohistochemical methods were used to detect the expression of CXCL8 protein in 4 imatinib resistant GIST and 4 sensitive GIST tissues. The Eighty-eight GIST patients were divided into CXCL8 high expression group(49 cases) and low expression group(39 cases), and the clinicopathological parameters were compared between the two groups of patients. Kaplan-Meier survival curve was drawn to compare overall survival(OS) and disease-free survival(DFS) in patients with high and low CXCL8 expression. Univariate and multivariate Cox regression models were used to analyze the factors affecting DFS. Results:Bioinformatics analysis showed that the expression of CXCL8 gene in drug-resistant GIST tissues was higher than that in sensitive tissues. Immunohistochemical results showed that the expression of CXCL8 protein in drug-resistant GIST tissues was significantly higher than that in sensitive tissues, with statistical significance(P<0.05). CXCL8 protein expression was correlated with the maximum tumor diameter, mitotic phase count, and risk stratification(P<0.05). Kaplan-Meier survival curve showed that DFS in patients with high CXCL8 expression was lower than that in patients with low CXCL8 expression(P<0.05). Cox regression model analysis showed that high expression of CXCL8 was an independent risk factor for DFS in GIST patients. Conclusion:CXCL8 promotes drug resistance of GIST and is associated with the prognosis of GIST patients, which may be a new target for specific treatment of gastrointestinal stromal tumors.更多还原