【摘要】 水稻卷叶突变体是研究叶片发育机制和培育理想株型品种的重要资源。本研究以1个可稳定遗传的卷叶突变体（暂命名为rl-z）为试验对象，对其开展表型鉴定和基因定位。表型鉴定结果显示，突变体rl-z的叶片在整个生育期均呈筒状内卷，与其野生型植株相比，突变体的株高、穗长、分蘖数、千粒重均显著下降。遗传分析结果表明，rl-z的突变表型受1对隐性核基因控制。利用突变体rl-z与籼稻品种扬稻6号杂交构建的F₂群体进行基因定位，将卷叶基因定位于第9染色体的InDel标记JY11至JY14之间，两标记之间物理距离为38.5 kb,此区间内仅有1个预测基因（RL9,LOC＿Os09g23200）,该基因编码GARP转录因子。对RL9进行测序，结果表明该基因在第1个外显子103 086～103 121处缺失36个碱基，同时在103 524处存在1个单碱基突变。基因定位结果说明突变体rl-z的卷叶特性可能是由于RL9基因突变导致GARP转录因子功能异常引起的。更多还原

【Abstract】 Rice rolled-leaf mutants are important resources for studying the mechanisms of leaf development and breeding desirable plant varieties. In this study, a rolled-leaf mutant（named rl-z） with stable inheritance was used as material to conduct phenotypic analysis and gene mapping. Phenotypic identification results showed that the leaves of rl-z mutants were inner rolled, forming a tube-like structure throughout the whole growth period. Compared with wild type rice, the plant height, panicle length, number of tillers, and thousand-grain weight of mutant were significantly decreased. Genetic analysis displayed that the mutant phenotype of rl-z was controlled by a pair of recessive genic genes. Gene mapping was performed by using the F₂ population produced by crossing rl-z mutant with an indica rice variety Yangdao 6. The rolled-leaf gene was mapped between the InDel markers JY11 and JY14 on chromosome 9, and the physical distance between the two markers was 38.5 kb. There was only one predicted gene（RL9, LOC＿Os09g23200） within the region, and this gene encoded a GARP transcription factor. Sequencing results showed that the first exon of RL9 was found to exist a 36 bp deletion（nucleotide position 103 086-103 121） and a single-base mutation（nucleotide position 103 524）. Results of gene mapping suggested that the rolled leaf characteristic of rl-z mutant was likely to be caused by the abnormal function of GARP transcription factor owing to the mutation of RL9.更多还原