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白城山新1号杨增殖培养试验
Multiplication Culture Experiment of Populus davidiana × P. bolleana ‘baichengshanxinyang No.1’
【摘要】 为筛选出白城山新1号杨最佳增殖培养基配方,本试验采用正交试验法经组织培养对其进行繁育,以MS培养基为基础配方,将6-BA质量浓度、NAA质量浓度、大量元素含量、蔗糖质量浓度分别设置5个水平进行试验。经回归分析,确定MS+6-BA 0.30 mg·L-1+NAA 0.05 mg·L-1+蔗糖20.00 g·L-1为白城山新1号杨组培增殖培养基优化配方。
【Abstract】 In order to screen out the best multiplication culture medium of Populus davidiana × P. bolleana ‘baichengshanxinyang No.1’, the orthogonal test method was used to breed it by tissue culture. Based on the MS culture medium, the mass concentration of 6-BA, the mass concentration of NAA, the content of macroelement and the mass concentration of sucrose were set at 5 levels respectively. Through regression analysis, MS+6-BA 0.30 mg·L-1+NAA 0.05 mg·L-1+sucrose 20.00 g·L-1 was determined as the optimal tissue culture.
【关键词】 白城山新1号杨;
组织培养;
增殖培养;
【Key words】 Populus davidiana × P.bolleana ‘baichengshanxinyang No.1’; tissue culture; multiplication culture;
【Key words】 Populus davidiana × P.bolleana ‘baichengshanxinyang No.1’; tissue culture; multiplication culture;
- 【文献出处】 吉林林业科技 ,Journal of Jilin Forestry Science and Technology , 编辑部邮箱 ,2024年01期
- 【分类号】S792.11
- 【下载频次】20