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白城山新1号杨增殖培养试验

Multiplication Culture Experiment of Populus davidiana × P. bolleana ‘baichengshanxinyang No.1’

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【作者】 王利张玉玲包颖岳龙孙鹏王新波

【Author】 WANG Li;ZHANG Yuling;BAO Ying;YUE Long;SUN Peng;WANG Xinbo;Forestry Science Academy of Baicheng City;Jilin Provincial Forestry Technology Popularizing Station;Forestry Bureau of Shulan City;

【通讯作者】 张玉玲;

【机构】 白城市林业科学研究院吉林省林业技术推广站舒兰市林业局

【摘要】 为筛选出白城山新1号杨最佳增殖培养基配方,本试验采用正交试验法经组织培养对其进行繁育,以MS培养基为基础配方,将6-BA质量浓度、NAA质量浓度、大量元素含量、蔗糖质量浓度分别设置5个水平进行试验。经回归分析,确定MS+6-BA 0.30 mg·L-1+NAA 0.05 mg·L-1+蔗糖20.00 g·L-1为白城山新1号杨组培增殖培养基优化配方。

【Abstract】 In order to screen out the best multiplication culture medium of Populus davidiana × P. bolleana ‘baichengshanxinyang No.1’, the orthogonal test method was used to breed it by tissue culture. Based on the MS culture medium, the mass concentration of 6-BA, the mass concentration of NAA, the content of macroelement and the mass concentration of sucrose were set at 5 levels respectively. Through regression analysis, MS+6-BA 0.30 mg·L-1+NAA 0.05 mg·L-1+sucrose 20.00 g·L-1 was determined as the optimal tissue culture.

  • 【文献出处】 吉林林业科技 ,Journal of Jilin Forestry Science and Technology , 编辑部邮箱 ,2024年01期
  • 【分类号】S792.11
  • 【下载频次】20
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