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杏仁皮缩合单宁酶法提取工艺优化

Optimization of extraction process for condensed tannins from almond skin by enzyme method

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【作者】 杜凯刘宇轩吕波马向荣李霄

【Author】 DU Kai;LIU Yuxuan;Lü Bo;MA Xiangrong;LI Xiao;College of Chemistry and Chemical Engineering,Yulin University;

【机构】 榆林学院化学与化工学院

【摘要】 在单因素基础上,利用响应面法优化杏仁皮缩合单宁的酶法提取工艺。以杏仁皮缩合单宁提取量为指标,考察了酶的用量、溶液pH值、提取温度、提取时间对杏仁皮缩合单宁提取量的影响。结果显示,所建立的杏仁皮缩合单宁提取工艺模型极显著,且在酶用量为26 U/g、溶液pH值为4.2、提取温度为52℃、提取时间为4 h的条件下,杏仁皮缩合单宁的提取量最高,预测值为38.71 mg/g,实际值为38.73 mg/g,结果表明优化的最佳提取工艺与预测方法相符。

【Abstract】 On the basis of single factor analysis, the enzymatic extraction process of condensed tannins from almond skin was optimized using response surface methodology.The effects of enzyme amount, solution pH,extraction temperature, and extraction time on the extraction amount of condensed tannins from almond skin were investigated using almond skin as an indicator.The results showed that the established process model for extracting condensed tannins from almond skin was extremely significant, and under the conditions of enzyme amount of 26 U/g, solution pH of 4.2,extraction temperature of 52 ℃,and extraction time of 4 h, the highest extraction amount of condensed tannins from almond skin was found, with a predicted value of 38.71 mg/g and an actual value of 38.73 mg/g.This indicates that the optimal extraction process for condensed tannins from almond skin using enzyme method was successfully established.

【关键词】 杏仁皮响应面缩合单宁酶法
【Key words】 Almond skinResponse surfaceCondensed tanninsEnzymatic method
【基金】 国家自然科学基金地区项目(52262015);榆林学院高层次人才科研启动基金项目(2023GK44);榆林学院横向项目(2022HX347);陕西省教育厅青年创新团队研究项目(23JP199);中国科学院洁净能源创新研究院-榆林学院联合基金项目(YLUDNL2021018);陕西省科技资源开放共享平台项目(2019PT-18);校企合作横向项目(2021HX161)
  • 【文献出处】 化工科技 ,Science & Technology in Chemical Industry , 编辑部邮箱 ,2024年03期
  • 【分类号】TS209;TS201.2
  • 【下载频次】20
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