【摘要】 为了同时对目标样本的三维空间结构及光谱信息进行分析，提出了基于Gabor模式的多光谱数字全息显微成像系统。即在Gabor同轴数字全息成像系统中引入可调谐多波段LED照明光源，获得目标样本在不同波长下的全息图像，通过采用角谱自聚焦和孪生像抑制算法获得目标样本在不同波长下的重建图像，估计样本透射光谱，实现像素级图像配准。使用美国空军（USAF）分辨率板对系统成像性能进行测试，选取不同生物组织样本及不同属性液体进行多光谱实验探究。结果表明，该系统成像分辨率可达7.81μm，不仅实现了彩色全息显微成像，具有良好的色彩再现像，还可以定性区分不同属性液体，在无标记生物样品的成像和检测领域中具有潜在的应用前景。更多还原

【Abstract】 To achieve simultaneous analysis of the three-dimensional spatial structure and spectral information of a target sample, a multi-spectral Gabor in-line digital holographic microscopy imaging system is proposed. Specifically, an adjustable multi-wavelength LED illumination source is introduced into the Gabor coaxial digital holographic imaging system. Holographic images of a target sample at different wavelengths are acquired, and the reconstructed images of the target sample at various wavelengths are obtained through angular spectrum autofocusing and twin-image suppression algorithms. This allows the estimation of the sample’s transmission spectra and realizes pixel-level image registration.The imaging performance of the system is tested using the United States air force(USAF) resolution chart, and multispectral experiments are conducted considering different biological tissue samples and liquids with different properties. The results demonstrate that the system can achieve an imaging resolution of up to 7. 81 μm. Furthermore, the proposed imaging technique not only achieves color holographic microscopy imaging with good color reproduction, but also qualitatively distinguish liquids with different properties, which has potential application prospects in the imaging and detection of unlabeled biological samples.更多还原