【摘要】 目的：观察补肾调经方对控制性超促排卵（controlled ovarian hyperstimulation, COH）小鼠甲状腺功能及卵巢储备的影响。方法：8～10周龄ICR系雌性小鼠随机分为正常组、COH组和补肾组，每组20只。COH组和补肾组采用由促性腺激素释放激素激动剂（GnRH-a）、人绝经期促性腺激素（HMG）和人绒毛膜促性腺激素（hCG）组成的COH方案，补肾组在给予以上方案的同时灌胃补肾调经方。放射免疫法（RIA）检测血清四碘甲腺原氨酸（T₄）、促甲状腺激素（TSH）、雌二醇（E₂）和孕酮（P）水平；ELISA检测血清抗缪勒氏管激素（AMH）水平；HE染色计数卵巢组织切片不同阶段卵泡。结果：与正常组相比，COH组小鼠血清T₄、AMH、E₂和P水平降低（P<0.05）,TSH水平升高（P<0.05）,黄体数增加，初级卵泡和次级卵泡数减少（P<0.05）;与COH组相比，补肾组小鼠血清T₄和AMH水平升高（P<0.05）,TSH浓度降低（P<0.05）,黄体数减少，初级卵泡和次级卵泡数增加（P<0.05）。结论：COH可能导致小鼠甲状腺功能障碍并降低卵巢储备，补肾调经方对改善COH小鼠甲状腺功能和卵巢储备发挥一定作用。更多还原

【Abstract】 Objective: To observe the effect of Bushen Tiaojing（kidney-nourishing and menstruation-regulating） prescription on thyroid function and ovarian reserve in mice with controlled ovarian hyperstimulation（COH）.Methods: Female ICR mice aged 8-10 weeks were randomly divided into a normal group, a COH group, and a kidney-tonifying group, with 20 mice in each group.Both the COH group and the kidney-tonifying group underwent a COH protocol consisting of gonadotropin-releasing hormone agonist（GnRH-a）,human menopausal gonadotropin（HMG）,and human chorionic gonadotropin（hCG）.In addition, the kidney-tonifying group was administered the Bushen Tiaojing prescription through gastric gavage concurrently with GnRH-a administration.Radioimmunoassay（RIA） was used to measure serum levels of thyroxine（T₄）,thyroid-stimulating hormone（TSH）,estradiol（E₂）,and progesterone（P）; ELISA was used to detect serum levels of anti-Müllerian hormone（AMH）; HE staining was performed to count follicles at different stages in ovarian tissue sections.Results: Compared with the normal group, the COH group mice showed decreased serum levels of T₄,AMH,E₂,and P（P<0.05）,increased TSH levels（P<0.05）,increased number of corpora lutea, and decreased numbers of primary and secondary follicles（P<0.05）; Compared with the COH group, the kidney-tonifying group mice showed increased serum levels of T₄ and AMH（P<0.05）,decreased TSH concentrations（P<0.05）,decreased number of corpora lutea, and increased numbers of primary and secondary follicles（P<0.05）.Conclusion: COH may lead to thyroid dysfunction and decreased ovarian reserve in mice, while the Bushen Tiaojing prescription plays a role in improving thyroid function and ovarian reserve in COH mice.更多还原