【摘要】 目的 对海洋来源的化合物?-卡拉胶进行体外抗肠道病毒71型活性研究。方法 细胞病变效应（CPE）抑制实验评价?-卡拉胶抗肠道病毒71型的活性，CPE实验探究?-卡拉胶的细胞毒性。通过空斑实验、Western blot实验、实时荧光定量PCR实验探究?-卡拉胶的抗病毒活性。利用Co-IP实验探究其作用靶点。结果 ?-卡拉胶具有高效低毒的抗肠道病毒71型活性，在Vero细胞中效果最好，半抑制浓度(IC₅₀)为6.29μg/mL，半数毒性浓度(CC₅₀)大于1 mg/mL，不同作用方式和不同作用时间实验表明，?-卡拉胶主要起作用于病毒吸附后阶段，且0～2 h给药可以显著抑制病毒VP1蛋白表达。此外，?-卡拉胶可以与病毒VP1蛋白结合，从而影响病毒感染细胞。结论 ?-卡拉胶具有良好的体外抗肠道病毒71型活性，为发现和改造新的海洋来源的糖类化合物提供了借鉴，为寻找新的抗肠道病毒71型药物提供了新思路。更多还原

【Abstract】 Objective In vitro studies on the anti-enterovirus 71 activity of the compound?-carrageenan of marine origin.Methods CPE inhibition assay to evaluate the activity of?-carrageenan against enterovirus 71,CPE assay to verify the mode of action and cytotoxicity of?-carrageenan,combined with empty spot assay,Western blot assay and real-time fluorescence quantitative PCR assay for corroboration.Co-IP assay was used to verify its target of action.Results?-carrageenan has a highly efficient and low-toxic anti-enterovirus71 activity and is most effective in Vero cells with a concentration of inhibitory 50%(IC₅₀) of 6.29μg/mL and a concentration of cytotoxicity 50%(CC₅₀) greater than 1 mg/mL.Experiments with different modes of action and different duration of action showed that?-carrageenan acted mainly in the post-adsorption phase of the virus,and 0-2 h administration could significantly inhibit viral VP1 protein expression.In addition,?-carrageenan could bind to the viral VP1 protein and thus affect virus-infected cells.Conclusion The good in vitro anti-enterovirus 71 activity of?-carrageenan provides insights into the discovery and modification of new glycoconjugates of marine origin and offers new ideas for the search for new anti-enterovirus 71 drugs.更多还原