【摘要】 目的 对一株软珊瑚来源共附生真菌Aspergillus versicolor CGF9-1-2的次生代谢产物进行研究。方法采用硅胶柱层析、十八烷基硅烷键合（ODS）柱层析、高效液相（HPLC）等色谱分离方法对其代谢产物进行分离纯化，运用高分辨质谱（HR-ESI-MS）、核磁共振（NMR）和旋光（ORD）等技术对分离得到的化合物进行结构鉴定，并对其进行DNA磷酸二酯酶1(TDP1)活性测试。结果 从Aspergillus versicolor CGF9-1-2中分离得到10个化合物，分别鉴定为(2S, 2′R, 3R, 3′E, 4E, 8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2′-hydroxyl-3′-octadecenoyl)amino-9-methyl-4, 8-octadecadiene(1)、(4E, 8E)-N-2′-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine(2)、desoxybrevianamide E(3)、cyclo (D-Pro-D-Phe)(4)、cyclo (L-Pro-D-Leu)(5)、indole-3-carboxylic-acid(6)、β-carboline(7)、(7S)-(+)-hydroxysydonic acid(8)、ent-aspergoterpenin C(9)和(E)-7-deoxy-7, 8-didehydro-12-hydroxysydonic acid(10)。TDP1活性测试结果表明，所有化合物均无明显抑制活性。结论 化合物2为首次从Aspergillus属真菌分离得到，化合物1为首次从Aspergillus versicolor真菌分离得到。更多还原

【Abstract】 Objective To study the chemical structures of secondary metabolites from soft coral derived fungus Aspergillus versicolor CGF9-1-2. Methods The secondary metabolites were separated and purified by silica gel column chromatography, octadecysilane(ODS) column chromatography, and semi-preparative HPLC. The structures of isolated compounds were subjected to high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR), and optical rotation dispersion(ORD) spectroscopy. They were also tested for their DNA phosphodiesterase 1(TDP1) activities. Results From the culture of Aspergillus versicolor CGF9-1-2, we successfully isolated ten known compounds and identified as(2S, 2′R, 3R, 3′E, 4E,8E)-1-O-(β-D-glucopyranosyl)-3-hydroxyl-2-(N-2′-hydroxyl-3′-octadecenoyl)amino-9-methyl-4, 8-octadecadiene(1),(4E, 8E)-N-2′-hydroxyoctade-canoyl-1-O-(β-D-glucopyranosyl)-9-methyl-4, 8-sphingadienine(2), desoxybrevianamide E(3), cyclo(D-Pro-D-Phe)(4), cyclo(L-Pro-D-Leu)(5), indole-3-carboxylic-acid(6), β-carboline(7),(7S)-(+)-hydroxysydonic acid(8), ent-aspergoterpenin C(9) and(E)-7-deoxy-7, 8-didehydro-12-hydroxysydonic acid(10). The results of TDP1 activity test showed that none of these compounds showed significant inhibitory activity. Conclusion Compound 2 was firstly isolated from Aspergillus, and compound 1 was obtained from Aspergillus versicolor for the first time.更多还原