【摘要】 啶虫脒（acetamiprid,AC）在环境中的蓄积可对生态系统和人体健康产生不利影响。本研究利用表面增强拉曼光谱（Surface-enhanced Raman spectroscopy,SERS）技术,构建了一种快速定性及定量检测环境水中啶虫脒的检测试剂盒。采用化学合成法制备了粒径为40～60 nm的纳米金溶胶作为SERS活性基底,以包覆铝箔的24孔板为反应平台,将一定量的聚沉盐负载到反应孔中诱导信号热点,并与便携式拉曼光谱仪集成为适用于现场检测的试剂盒。通过对聚沉盐负载量、聚沉时间等条件进行优化,在浓度为25～750 ng·mL^-1范围内,啶虫脒浓度与SERS信号强度呈现良好的线性关系,R²=0.988。试剂盒检出限低至15.56 ng·mL^-1,平均加标回收率为90.04%～106.10%,在5 min内实现了环境水中啶虫脒的现场检测。与传统方法相比,该试剂盒便携、操作简单、快速、成本低廉、可同时进行多样本检测,为环境水体中啶虫脒的现场检测分析提供了新选择。更多还原

【Abstract】 The accumulation of acetamiprid（AC） in the environment can adversely affect ecosystems and human health.This study constructed a portable kit for the rapid qualitative and quantitative detection of AC in environmental water.Gold colloid with a 40-60nm particle size was prepared using the chemical synthesis method and used as the SERS substrate.An aluminum foil was placed on a 24-hole plate to serve as a bearing platform.Additionally,a certain amount of aggregation salt was loaded to induce signal hot spots.All the SERS signals were collected using a portable Raman spectrometer.In this study,aggregating conditions,such as the type and concentration of salts,were optimized.A linear relationship between AC concentration and SERS’s signal strength was observed,with an R² value of 0.988.The detection limit of this kit was calculated to be 15.56 ng·mL^-1.The average recovery rate ranged from 90.04%to 106.10%.Compared with the traditional method,the kit is portable,simple to operate,rapid,and inexpensive.It can detect multiple samples simultaneously,providing a new alternative for the field detection of AC in environmental water.更多还原