【摘要】 酶的米氏常数（K_m）测定是化学生物学实验教学中的经典项目，不仅锻炼学生的动手能力，还可以提高学生的逻辑思维能力。米氏常数K_m测定需要先得到反应初速率。反应初速率可通过朗伯比尔定律或者反应产物的浓度-吸光度标准曲线获得。通过对这两种方法的比较，发现通过浓度-吸光度标准曲线测定反应初速度的过程，是学生最容易理解，也是学生取得实验结果较好的方法，极大的提高了米氏常数K_m测定的教学质量。更多还原

【Abstract】 The measurement of the enzyme Michaelis constant(K_m) is a classic project of chemical biology teaching experiments, which exercises students’ practical ability and improves students’ logical thinking ability. The initial reaction rate must be obtained first to measure the Michaelis constant K_m. The initial reaction rate can be obtained by Lamberbier’s law or the concentration-absorbance standard curve of the product. Through the comparison of the two methods, it is found that the initial reaction rate obtained through the concentrationabsorbance standard curve is easier for students to understand and is the better way for students to obtain satisfied experimental results, which greatly improves the teaching quality of the measurement of Michaelis constant K_m.更多还原