【摘要】 目的 分析碳酸酐酶9(CA9)在口腔鳞状细胞癌(OSCC)组织中的表达与临床病理特征的相关性及其预后诊断价值。方法 采用定量反转录聚合酶链反应(qRT-PCR)检测CA9 mRNA在5对新鲜OSCC组织和癌旁组织中的表达。通过免疫组化检测CA9蛋白在86对配对的OSCC组织和癌旁组织中的表达水平，并探讨CA9的表达水平与患者临床病理参数的相关性。通过Kaplan-Meier生存分析法探讨CA9表达水平与OSCC预后的相关性，采用Cox回归分析影响OSCC预后的因素。利用肿瘤基因组图谱(TCGA)数据库分析CA9在OSCC组织中的表达及其预后诊断价值。结果 CA9在OSCC组织中的表达高于癌旁组织(P<0.05),与TCGA数据库分析结果一致，且其表达高低与淋巴结转移、临床分期相关(P<0.05);CA9高表达OSCC患者的总生存期短于CA9低表达组(P<0.05);淋巴结转移、CA9表达水平是影响OSCC患者预后的独立预测因素(P<0.05);CA9可能对OSCC有一定诊断意义(P<0.01)。结论 CA9在OSCC中高表达，且与OSCC预后不良有关，对OSCC可能具有一定的诊断价值。更多还原

【Abstract】 Objective To analyze the expression of carbonic anhydrase 9(CA9) in oral squamous cell carcinoma(OSCC) tissues and its correlations with the clinicopathological features and its prognosis value.Methods The expression levels of CA9 mRNA in 5 pairs of fresh OSCC tissues and adjacent normal tissues were detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR). The expression levels of CA9 in 86 paired paraffin-embedded OSCC tissues and adjacent normal tissues were detected by immunohistochemistry. The correlation of CA9 expression with the clinicopathological features was analyzed. Kaplan-Meier survival curve was used to analyze the relationship between the expression of CA9 and the prognosis of OSCC, and Cox model was used to analyze the factors affecting the prognosis of OSCC. The expression of CA9 in OSCC tissues and its prognostic diagnostic value were detected by The Cancer Genome Atlas(TCGA) database.Results The expression of CA9 in OSCC tissues was higher than that in para-carcinoma tissues(P<0.05), which was consistent with the results of TCGA database analysis, and its expression was closely related to lymphatic metastasis and clinical stage(P<0.05). The overall survival of OSCC patients with high CA9 expression group was shorter than patients with low CA9 expression(P<0.05). Lymphatic metastasis and CA9 expression were independent predictor of the prognosis of OSCC patients(P<0.05). CA9 may have some diagnostic value for OSCC(P<0.01).Conclusion CA9 is highly expressed in OSCC and is associated with unfavorable prognosis of the patients, and may have some diagnostic value for OSCC.更多还原