【摘要】 为获得具有杀松材线虫活性的菌株，在湖南省浏阳市采集了健康马尾松、感染松材线虫病但尚未死亡的马尾松以及感染松材线虫病致死的马尾松材料，从中分离得到了27株内生细菌进行杀线活性的测定，并对高效拮抗菌株进行了鉴定。结果表明：从感染但尚未死亡的松树分离得到的细菌H-4都具有较高的杀线活性；在发酵液处理48 h后，菌株H-4的杀松材线虫活性最高，松材线虫校正死亡率和消解率分别达到97.1%和89.2%。综合考虑菌株H-4的形态学特征、生理生化测定结果以及16S rRNA系统发育树分析结果，确定菌株H-4为暹罗芽孢杆菌。更多还原

【Abstract】 To obtain the strains capable of killing pine wood nematodes,we collected the healthy plants,the plants infected with pine wood nematodes but still alive,and the plants infected with pine wood nematodes and dead of Pinus massoniana from Liuyang City,Hunan Province.A total of 27 strains of endophytic bacteria were isolated and subjected to assessment of their nematocidal effects.Furthermore,the efficient antagonistic strains were identified.The results showed that strain H-4 isolated from the infected but still alive plants exhibited a high nematocidal activity.The treatment with the fermentation broth of strain H-4 for 48 h achieved the highest killing effect on pine wood nematodes,with the corrected mortality and clearance rates of pine wood nematodes reaching 97.1% and 89.2%,respectively.Strain H-4 was identified as Bacillus siamensis according to the morphological,physiological,and biochemical characteristics and the phylogenetic tree built based on the 16S rRNA sequence.更多还原