【摘要】 为促进猕猴桃功能基因组学的研究，以‘红阳’猕猴桃愈伤组织为试验材料，用10、25、50、75、100、150、200、300、400、500Gy共10个不同辐射剂量的⁶⁰Co–γ射线处理愈伤组织，以0Gy为对照，对诱变后代的表型变异特征进行分析。结果表明：经100 Gy剂量诱变处理后，‘红阳’猕猴桃愈伤组织的致变率已提升至35.9%，同时分化率亦达到77.8%；基于此，筛选出100 Gy为该品种愈伤组织的适当诱变条件；用100 Gy诱变条件处理1680块‘红阳’猕猴桃愈伤组织，构建了由1026株再生苗组成的‘红阳’猕猴桃突变体库，该突变体库在叶片、株型等性状上均存在丰富的变异，总变异频率达35.9%。更多还原

【Abstract】 To accelerate the development of functional genomic research, the mutant library of ‘Hongyang’ kiwifruit was constructed. The callus of ‘Hongyang’ kiwifruit were treated with ⁶⁰Co-γ rays at 10 distinct radiation doses: 10, 25, 50,75, 100, 150, 200, 300, 400, and 500 Gy, with 0 Gy serving as the control, and the phenotypic variation characteristics of the mutagenic progeny were analyzed. The results indicated that after mutagenic treatment with a dose of 100 Gy, the mutation rate of callus tissue from ‘Hongyang’ kiwifruit increased to 35.9%, while the differentiation rate reached 77.8%.Based on these findings, a radiation dose of 100 Gy was selected as the further studied condition.After treating 1 680‘Hongyang’ kiwifruit callus under optimal mutagenesis conditions, a kiwifruit mutant library contained 1 026 regenerated seedlings of ‘Hongyang’ kiwifruit was constructed. The mutant library had rich variation in leaf, plant type and other characters, with a total variation frequency of 35.9%.更多还原