【摘要】 为了探究FBXL3基因在驴卵巢、卵泡液和乳腺的表达水平与驴发情期和乏情期之间的关系，试验以德州毛驴为研究对象，利用qPCR方法分析FBXL3基因在发情期和乏情期不同组织中的表达情况，利用生物信息学方法预测FBXL3蛋白质的理化性质、跨膜结构域、亚细胞定位、蛋白质相互作用情况、二级结构和三级结构。结果表明：FBXL3基因在发情期卵巢和卵泡液中的相对表达量极显著高于乏情期(P<0.01),乳腺中的相对表达量显著高于乏情期(P<0.05)。FBXL3蛋白有37个酶解位点，属于亲水性蛋白，无跨膜结构域，主要分布在线粒体上，为酸性、不稳定蛋白质；FBXL3蛋白中α-螺旋、β-转角、无规则卷曲和延伸链分别占49.30%、2.57%、35.98%和12.15%,与三级结构预测结果一致；SKP1、FBXL13、CRY1、CRY2、PER1、PER2等蛋白与FBXL3蛋白存在相互作用。说明FBXL3基因在发情期和乏情期的表达具有组织特异性。更多还原

【Abstract】 In order to investigate the expression levels of FBXL3 gene in donkey ovary, follicular fluid and mammary gland, and its relationship with donkey estrus and anestrus stages, in the experiment, Dezhou donkeys were used as the research object; qPCR method was used to analyze the expression of FBXL3 gene in different tissues during estrus and anestrus stages; bioinformatics methods were used to predict the physicochemical properties, transmembrane structural domains, subcellular localization, protein interactions, secondary and tertiary structures of the protein. The results showed that the difference in the expression of FBXL3 gene was highly significant(P<0.01) in ovarian and follicular fluid during estrus and anestrus stages, and significant(P<0.05) in mammary gland. FBXL3 protein had 37 enzymatic sites and was a hydrophilic protein; it had no transmembrane structural domains and was mainly distributed on mitochondria, and it was an acidic and unstable protein. The α-helix, β-turn, irregularly coiled and extended chains in FBXL3 protein accounted for 49.30%, 2.57%, 35.98% and 12.15%, respectively, which was consistent with the tertiary structure prediction. Proteins such as SKP1, FBXL13, CRY1, CRY2, PER1 and PER2 interacted with FBXL3 protein. The results indicated that the expression of FBXL3 gene in estrus and anestrus stages was tissue-specific.更多还原